The Advantage of Automatic Control in Human-Like Collagen Production by Recombinant Escherichia coli BL21

Ting Zhen Mu; Yan'e Luo; Dai Di Fan; Tao Zhang

doi:10.4028/www.scientific.net/AMR.535-537.2316

Paper Titles

Synthesis and Characterization of Carboxymethyl Chitosan/Human-Like Collagen Hydrogel
p.2296

Study on the Bio-Composite from Sisal Fiber Reinforced Cellulose Acetate
p.2301

Preparation of Transglutaminase Cross-Linked Tilapia Scale Collagen Microspheres Containing L-Ascorbic Acid
p.2307

The High-Cell-Density Fermentation of Recombinant Escherichia coli Based on the Control System
p.2312

The Advantage of Automatic Control in Human-Like Collagen Production by Recombinant Escherichia coli BL21
p.2316

Structure of Antheraea pernyi/Bombyx mori Silk Fibroin Scaffolds
p.2321

Sodium Alginate Extraction by Enzyme-Ultrasonic Combined Method
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Optimization of the Extraction of Coconut Protein and the Evaluation of Antioxidant and ACE Inhibitory Activities of its Enzymatic Hydrolysates
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Chemical Analysis and Antioxidant Activities In Vitro of Polysaccharide Extracted from Corn Silk
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HomeAdvanced Materials ResearchAdvanced Materials Research Vols. 535-537The Advantage of Automatic Control in Human-Like...

The Advantage of Automatic Control in Human-Like Collagen Production by Recombinant Escherichia coli BL21

Abstract:

Two control models, manual way and automatic way, have been compared during the fermentation process in this study. The automatic way is achieved by a fermentation controller developed by ourselves. Compared to manual way, biomass (OD₆₀₀) and the production of human-like collagen (HLC) could be increases significantly, reaching to 144 and 7.12g/L respectively. Acetic acid, a key byproduct, is also lowered very obviously. Beside the production controlled by automation model was very stable, the automatic controller can regulate the feeding rate in real time and save labor force.

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Periodical:

Advanced Materials Research (Volumes 535-537)

Pages:

2316-2320

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.535-537.2316

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Online since:

June 2012

Authors:

Ting Zhen Mu, Yan'e Luo*, Dai Di Fan, Tao Zhang

Keywords:

Automatic Controller, Escherichia coli, Fermentation, Human-Like Collagen

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* - Corresponding Author

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