Preparation of Transglutaminase Cross-Linked Tilapia Scale Collagen Microspheres Containing L-Ascorbic Acid

Peng Li; Xuan Ri Shen; Zhou Wei Duan; Zhang Han Wang

doi:10.4028/www.scientific.net/AMR.535-537.2307

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Study on the Bio-Composite from Sisal Fiber Reinforced Cellulose Acetate
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The Advantage of Automatic Control in Human-Like Collagen Production by Recombinant Escherichia coli BL21
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HomeAdvanced Materials ResearchAdvanced Materials Research Vols. 535-537Preparation of Transglutaminase Cross-Linked...

Preparation of Transglutaminase Cross-Linked Tilapia Scale Collagen Microspheres Containing L-Ascorbic Acid

Abstract:

With the goal to develop biocompatible material collagen extracted from tilapia scale as coating material to encapsulate bioactive compounds, a microsphere containing L-ascorbic acid as a model is developed, using natural nontoxic transglutaminase (TG) as cross-linking agent. Ultrasonic wave was applied in the process to assist preparing microcapsule. Factors of emulsifier, TG, ratio of L-ascorbic acid and collagen, incubation temperature were discussed. The formulation from orthogonal experiment was of the optimal encapsulation efficiency 81.17%.It is found that the collagen extracted from tilapia scale is a potential biomaterial.

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Periodical:

Advanced Materials Research (Volumes 535-537)

Pages:

2307-2311

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.535-537.2307

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Online since:

June 2012

Authors:

Peng Li*, Xuan Ri Shen, Zhou Wei Duan, Zhang Han Wang

Keywords:

Biomaterial, Collagen, L-Ascorbic Acid, Microencapsulation, Tilapia, Transglutaminase

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