Preparation and Properties of Sensing Membrane for Fiber Optic DNA Sensor

Hua Fang; You Rong Wang

doi:10.4028/www.scientific.net/AMR.542-543.620

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HomeAdvanced Materials ResearchAdvanced Materials Research Vols. 542-543Preparation and Properties of Sensing Membrane for...

Preparation and Properties of Sensing Membrane for Fiber Optic DNA Sensor

Abstract:

To detect DNA concentration, a novel fiber optic biosensor based on fluorescence quenching was designed and constructed by using lock-in amplifier for signal measurement. The sensor head was fixed on a sensing membrane prepared by the mixture of ethyl silicate, formamide, HCl and fluorescence indicator [Ru(phen)₃]²⁺. The optimal film-forming conditions were that the contents of [Ru(phen)₃]Cl₂, HCl and formamide were 2.1 mg/ml, 12 % and 16 % respectively, the aging temperature and time of sol-gel solution were 4 oC and 5 d, and the film-forming temperature and time were 4 oC and 48 h, respectively. A linear relationship between the relative phase delay Δφ of sensing membrane and ctDNA concentration was observed in range from 2.0×10^-9 to 4.0×10^-7 M in Tris buffer, and the detection limit is 8.0×10^-10 M. The biosensor had a response time of 65 s and showed good stability and repeatability.

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Advanced Materials Research (Volumes 542-543)

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620-624

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https://doi.org/10.4028/www.scientific.net/AMR.542-543.620

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Online since:

June 2012

Authors:

Hua Fang, You Rong Wang

Keywords:

ctDNA, Fluorescence Quenching, Lock-In Amplifier, Sensing Membrane

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