Preparation of Cross-Linked Cellulase Aggregates onto Magnetic Chitosan Microspheres

Xiao Ling Xie; Bing Li; Zhi Qing Wu; Shou Li Dong; Lin Li

doi:10.4028/www.scientific.net/AMR.550-553.1566

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HomeAdvanced Materials ResearchAdvanced Materials Research Vols. 550-553Preparation of Cross-Linked Cellulase Aggregates...

Preparation of Cross-Linked Cellulase Aggregates onto Magnetic Chitosan Microspheres

Abstract:

The cellulase was immobilized onto magnetic chitosan microspheres carrier as cross-linked enzymes aggregates (CLEAs). It was precipitated with 95% saturation ammonia sulfate and cross-linked with 3% (v/v) glutaraldehyde. Efficient enzyme activity about 50.6% was obtained when cellulase concentration was 1.0mg/mL after cross-linking for 7 h at 30^○C. The CLEAs was advantageous on stabilities and magnetic responsiveness for separation.

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Periodical:

Advanced Materials Research (Volumes 550-553)

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1566-1571

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https://doi.org/10.4028/www.scientific.net/AMR.550-553.1566

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Online since:

July 2012

Authors:

Xiao Ling Xie, Bing Li, Zhi Qing Wu, Shou Li Dong, Lin Li

Keywords:

Cellulase, Cross-Linked Enzyme Aggregates, Magnetic Chitosan Microspheres

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