Optimization of Sargassum Extracts Preparation with Complex Enzyme by Response Surface Methodology

Shu Xing Liu; Shuang Zhang; Wei Na Jiao

doi:10.4028/www.scientific.net/AMR.550-553.1653

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HomeAdvanced Materials ResearchAdvanced Materials Research Vols. 550-553Optimization of Sargassum Extracts Preparation...

Optimization of Sargassum Extracts Preparation with Complex Enzyme by Response Surface Methodology

Abstract:

Use Design Expert software to establish a quadratic polynomial model between four factors and content of reducing sugar which is characterization for hydrolysis. The four factors are the amount of complex enzyme, the ratio of complex enzyme, hydrolysis temperature and hydrolysis time. Process of sargassum extracts preparation with complex enzyme is optimized by response surface methodology. The optimum preparation conditions were achieved as follows：amount of complex enzyme is 2.05%, ratio of complex enzyme is 1.98, hydrolysis temperature is 55°C, hydrolysis time is 64minutes, and the final reducing sugar content is 0.832% which is good repeatability.

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Periodical:

Advanced Materials Research (Volumes 550-553)

Pages:

1653-1658

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.550-553.1653

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Online since:

July 2012

Authors:

Shu Xing Liu, Shuang Zhang, Wei Na Jiao

Keywords:

Cellulase, Pectinase, Response Surface, Sargassum Extracts

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