Study on Macroporous Resin Separation and Purification of Total Flavonoids of Plantago Process

Article Preview

Abstract:

To study the macroporous resin separation and purification of total flavonoids of Plantago optimum conditions, the static adsorption and desorption experiments by filtering out the best macroporous resin, and plantain extract for the assessment of total flavonoid content indicators in the single-factor test based on the orthogonal design, the amount of resin in the hole, eluent concentration, alcohol consumption and other factors for the study to optimize the AB-8 macroporous resin separation and purification of total flavonoids plantain best process conditions. The results show that: the effect of various factors affecting the size of the order of separation and purification has to: eluent concentration> ethanol consumption> macroporous resin usage, optimization of the optimum conditions: resin dosage is 1 times the amount of crude drug, the use of eluent volume fraction of 40% ethanol, elution agent is 12.5 times the amount of crude drug. Verify the conditions in this test, three repeated experiments were similar, the average flavonoid content 0.2127%, the transfer rate of 90.57%, indicating that the scientific and rational and stable process conditions can be effectively used plantain purified flavonoids.

You might also be interested in these eBooks

Info:

Periodical:

Advanced Materials Research (Volumes 550-553)

Pages:

987-992

Citation:

Online since:

July 2012

Export:

Price:

Permissions CCC:

Permissions PLS:

Сopyright:

© 2012 Trans Tech Publications Ltd. All Rights Reserved

Share:

Citation:

[1] Ren Yijun, Zhou Hai,Yang YuanRong,et al.Journal of Research Situation of Plantain[J].Anhui Agri.Sci.2009,37(18):8467-8469

Google Scholar

[2] Liu Lifeng.Research Situation of Plantain[J]. Guide of China Medicine, 2009,7(5):40-41.

Google Scholar

[3] Nishibe S, Tomayama Y, Sasahara M, et al. Aphenyl ethanoidglycoside from plantago asiatica[J]. Phytochem, 1995, 38(3):741-742.

Google Scholar

[4] Chen Xu, Lan Luo, Ren Xiang. Tan A ntidep ressant effect of th ree traditional Chinese medicines in the learned help lessnessmodel[J].Journal of Ethnopharmacology, 2004, 91: 345-349.

DOI: 10.1016/j.jep.2004.01.012

Google Scholar

[5] Wu Caie,Fang Shengzuo,Feng Zhuoshuai, et al.Purification Process of Total flavonoids in Cyclocarya paliurus Leaves with Macroporous resins[J].Transactions of the Chinese Society for Agricultural Machinery, 2009,40(6): 133-134.

Google Scholar

[6] GUO Li-mei, BAI Ming, YAO Pei-zheng, et al. Optimization of Separation and Purification of Total Flavonoids from the Pine Pollen by Macroporous Resin[J].Modern Food Science and Technology,2009,25(2):162-163.

Google Scholar