Anti-Proliferative Activity of Glycoprotein PLG-3 from the Submerged Mycelia of Phellinus linteus AML1101 In Vitro

Abstract:

A novel glycoprotein PLG-3 was obtained from the cultured mycelia of the edible mushroom Phellinus linteus AML1101 by isolating with cold water for 24 h and fractioned with DEAE-Sephrose Fast Flow column. It exhibited potent antiproliferative activity on A549 cells and HepG2 cells in vitro. After 24, 48, 72- h treatment with PLG-3, IC50 values of A549 cells and HepG2 cells were 45.77, 24.09, 16.54 μg/L and 47.78, 26.73, 18. μg/L, respectively. PLG-3 had a molecular weight of 2.5×104 Da, and consisted of approximately 85.5% of protein and 13.7% polysacchrides. It was composed of arabinose, xylose, mannose, glucose and galactose with a molar ratio of 1.46: 2.22: 7.74: 1.29:0.61.The obtained results proven that PLG-3 was a protein-dominant glycoprotein with singificant antiproliferative activity.

• Periodical: Advanced Materials Research (Volumes 554-556)
• Pages: 1140-1147
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.554-556.1140
• Online since: July 2012
• Authors: Feng Jie Cui, Xin Yi Zan, Hong Yuan Wang, Wen Jing Sun, Da Ming Huang, Zhi Cai Zhang
• Keywords: Anti-Proliferative Activity, Glycoprotein, Mycelia, Phellinus linteus

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