Characterization of the Dynamic Changes of Microorganisms in Cutlassfish (Trichiurus haumela) under the Cold Storage with Composite Natural Preservatives Based on Culture-Dependent and 16S rRNA-DGGE Technology

Abstract:

In this study, the main microbe dynamic changes of cutlassfish (Trichiurus haumela) treated with composite natural preservatives under the cold storage (4±1) °C were studied by the methods of culture-dependent and denaturing gradient gel electrophoresis (DGGE) fingerprinting analysis based on the sequence of 16S rRNA V3 region gene, which provided the theory basis and reference to the composite natural preservatives’ mechanism and extended the shelf-life of aquatic products. The results showed that 13 kinds of bacteria were identified by the culture-dependent methods, the dominant bacteria belonged to Shewanella putrefaciens and Pseudomonas fluorescens. By sequencing analysis, 12 kinds of bacteria in main DGGE spectra stripe of cutlassfish. In the later periods, the specific spoilage organism (SSO) for the treatment group with composite natural preservatives and the controlled of cutlassfish were highly similar. Psychrobacter sp. was the main bacterium in the initial stage of the storage. With the extension of storage time, the proportion of Shewanella sp. and Pseudomonas sp. increased gradually and they took the place of Psychrobacter sp. to be the dominant bacteria in the process of storage. Thereinto, Pseudomonas fluorescens and Vibrio sp. both took high proportions in the process of storage. At the same time, composite natural preservatives had an obvious growth inhibition effects on the bacteria of cutlassfish such as Shewanella sp. and Pseudomonas sp..

• Periodical: Advanced Materials Research (Volumes 554-556)
• Pages: 1498-1506
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.554-556.1498
• Online since: July 2012
• Authors: Wei Qing Lan, Jing Xie
• Keywords: Cold Storage, Composite Natural Preservatives, Culture-Dependent, Dynamic Change, PCR-DGGE, Trichiurus haumela

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