Biotransformation from Isoeugenol to Vanillin by Immobilized Bacillus fusiformis CGMCC1347 Cells

Abstract:

A novel strain, Bacillus fusiformis CGMCC1347, was utilized successfully to transform isoeugenol to vanillin and the product inhibition could be well avoided by using immobilized cells in the isoeugenol/aqueous biphasic system. The Bacillus fusiformis CGMCC1347 cells were entrapped into sodium alginate under conditions of 2.5% sodium alginate, 10% cells and 0.1 mol l-1 CaCl2. The optimum pH for free cells and immobilized cells were 4.0 and 3.5, respectively, and the optimum reaction temperature for both free and immobilized cells was 37 °C. The pH stabilities of free cells and immobilized cells were good at all investigated pH levels. For thermal stability, free cells were stable at 50°C60°C and the immobilized cells were stable at 50°C 80°C. The half-life of the immobilized cells was more than 25 d, comparing with less than 14 d for the free cells at 4°C.

• Periodical: Advanced Materials Research (Volumes 554-556)
• Pages: 1507-1510
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.554-556.1507
• Online since: July 2012
• Authors: Li Qing Zhao, Jia Mao Fang, Xiang Dong Xiao
• Keywords: Bacillus fusiformis CGMCC1347, Biotransformation, Cells Immobilization, Isoeugenol, Reuse, Vanillin

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