Vector-Based siRNA is a Powerful Tool to Reduce Protein Expression in Cancer Research

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Abstract:

RNAi become a popular technique in exploring gene function. Synthesized siRNA duplexes and vector-based siRNA are mostly used in gene silencing. However, vector-based siRNA constructed from plasmids are rarely used in inhibition gene expression.In our study, vector-based siRNA are used in reducing PSMD4 expression. Our study suggests that vector-based siRNA can be used in cancer research.

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Advanced Materials Research (Volumes 554-556)

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1747-1750

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July 2012

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© 2012 Trans Tech Publications Ltd. All Rights Reserved

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[1] Fire, A. RNA-triggered gene silencing. Trends Genet. Vol 15, 358-363 (1999)

Google Scholar

[2] Sharp, P. A. RNA interference 2001. Genes Dev. Vol 15, 485-490 (2001).

Google Scholar

[3] Hammond, S. M., Caudy, A. A. & Hannon, G. J. Post-transcriptional gene silencing by doublestranded RNA. Nature Rev. Genet. Vol 2, 110-1119 (2001).

DOI: 10.1038/35052556

Google Scholar

[4] Tuschl, T. RNA interference and small interfering RNAs. Chem. Biochem. Vol 2, 239-245 (2001).

Google Scholar

[5] Hamilton, A. J. & Baulcombe, D. C. A species of small antisense RNA in posttranscriptional gene silencing in plants. Science Vol 286, 950-952 (1999)

DOI: 10.1126/science.286.5441.950

Google Scholar

[6] Hammond, S. M., Bernstein, E., Beach, D. & Hannon, G. J. An RNA-directed nuclease mediates posttranscriptional gene silencing in Drosophila cells. Nature Vol 404, 293-296 (2000).

DOI: 10.1038/35005107

Google Scholar

[7] Zamore, P. D., Tuschl, T., Sharp, P. A. & Bartel, D. P. RNAi: Double-stranded RNA directs the ATPdependent cleavage of mRNA at 21 to 23 nucleotide intervals. Cell Vol 101, 25-33 (2000).

DOI: 10.1016/s0092-8674(00)80620-0

Google Scholar

[8] Bernstein, E., Caudy, A. A., Hammond, S. M. & Hannon, G. J. Role for a bidentate ribonuclease in the initiation step of RNA interference. Nature Vol 409, 363-366 (2001).

DOI: 10.1038/35053110

Google Scholar

[9] Elbashir, S. M., Lendeckel,W. & Tuschl, T. RNA interference is mediated by 21 and 22 nt RNAs. Genes Dev. Vol 15, 188-200 (2001).

DOI: 10.1101/gad.862301

Google Scholar

[10] Caplen, N. J., Fleenor, J., Fire, A. & Morgan, R. A. dsRNA-mediated gene silencing in cultured Drosophila cells: a tissue culture model for the analysis of RNA interference. Gene Vol 252, 95-105 (2000).

DOI: 10.1016/s0378-1119(00)00224-9

Google Scholar

[11] Clemens, J. C. et al. Use of double-stranded RNA interference in Drosophila cell lines to dissect signal transduction pathways. Proc. Natl Acad. Sci. USA Vol 97, 6499-6503 (2000).

DOI: 10.1073/pnas.110149597

Google Scholar

[12] Devroe E, Silver PA 2004. Therapeutic potential of retroviral RNAi vectors. Expert Opin Biol Ther, Vol 4, 319-27.

DOI: 10.1517/14712598.4.3.319

Google Scholar

[13] Elbashir SM, Martinez J, Patkaniowska A, Lendeckel W, Tuschl T. 2001a. Functional anatomy of siRNAs for mediating efficient RNAi in Drosophila melanogaster embryo lysate. EMBO J, Vol 20, 6877-6888.

DOI: 10.1093/emboj/20.23.6877

Google Scholar

[14] Elbashir SM, Harborth J, Lendeckel W, Yalcin A, Weber K,Tuschl T. 2001b. Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells. Nature, Vol 24, 494-498

DOI: 10.1038/35078107

Google Scholar