Water Caltrop Pericarps Extracts Attenuate H2O2-Induced Human Umbilical Vein Endothelial Cell Injury

Qiu Sheng Lin; Hai Ning Yu; Undurti N Das; Sheng Rong Shen

doi:10.4028/www.scientific.net/AMR.554-556.1757

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HomeAdvanced Materials ResearchAdvanced Materials Research Vols. 554-556Water Caltrop Pericarps Extracts Attenuate...

Water Caltrop Pericarps Extracts Attenuate H₂O₂-Induced Human Umbilical Vein Endothelial Cell Injury

Abstract:

Natural antioxidants could inhibit ROS production and attenuate injury, which is important event of cardiovascular diseases. Water caltrop pericarps from three different Chinese water caltrop cultivars were collected and extracted using 70% methanol and hot water (WCPE). WCPE exhibited strong in vitro antioxidant activities tested by DPPH assay. Incubation with 100μM H2O2 for 1 h would reduce viability of HUVECs to 76.46±0.05%, decrease SOD, CAT and GSH-Px activities to 12.50±0.06, 10.08±0.57 and 107.98±2.68 U/mg protein compared with 20.57±0.02, 22.56±0.41 and 215.28±23.13 U/mg protein of normal cells, increase MDA content from 3.31±0.38 to 9.52±1.87 nmol/mg protein. Pre-treatment, post-treatment and simultaneous with WCPE could attenuate HUVECs injury and recover the viability to 107.77±0.06%, SOD, CAT and GSH-Px activities to 19.11±0.02, 26.14±0.89 and 192.62±7.75 U/mg protein and MDA content to 4.31±0.71 nmol/mg protein. These results suggested that WCPE attenuates H2O2-induced HUVECs injury through promoting proliferation, enhance antioxidant enzymes activities and reduce lipid peroxidation.

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Periodical:

Advanced Materials Research (Volumes 554-556)

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1757-1761

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.554-556.1757

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Online since:

July 2012

Authors:

Qiu Sheng Lin, Hai Ning Yu, Undurti N Das, Sheng Rong Shen

Keywords:

H₂O₂, Human Umbilical Vein Endothelial Cell, Injury, Water Caltrop Pericarp

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