Rapid Analysis of the Main Components of Glycyrrhizae Uralensis Fisch by UPLC/Q-TOF-MS

Abstract:

A rapid method for analysis of the main components of Radix Glycyrrhizae (RG) was developed using ultra-performanceliquid chromatography with quadrupole-time-of-flight mass spectrometry (UPLC/Q-TOF-MS). Previous study demonstrated that RG had antivirus, antioxidant，anti-tumor and other bioactivities. The separation analysis was performed on Waters Acquity UPLC system and the accurate mass of molecules and their fragment ions were determined by Q-TOF MS. Fourty compounds including triterpenoid saponins, flavonoid glycosides and flavonoid aglycones,coumarins, were identified and tentatively deduced on the basis of their element compositions, MS/MS data and relevant literatures. The results demonstrated that triterpenoid saponins, flavonoid glycosides and flavonoid aglycones were the main constituents of RG. Furthermore, an effective and rapid pattern was established allowing for comprehensive and systematic characterization of the complex samples.

• Periodical: Advanced Materials Research (Volumes 581-582)
• Pages: 269-272
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.581-582.269
• Online since: October 2012
• Authors: Zhong Feng Shi, Yi Fan Feng, Wen Rui, Liang Wang, Xiao Mei Zhang, Liang Han
• Keywords: MS/MS, Radix Glycyrrhizae, Rapid Analysis, UPLC/Q-TOF

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