Analytical Method of Gliotoxin Content by HPLC

Article Preview

Abstract:

Objective: To establish a determination method for gliotoxin. Methods: Reverse-phase high performance liquid chromatography (HPLC) method was used:the column was Inertsil ODS-SP; detection wavelength set at 254 nm; mixed solution of menthol and water(50:50)was used as the mobile phase with flow rate of 1.0 mL/min. Results: The regression equation of gliotoxin content was y = 9E–08x–0.003. The linear range was 0.5-2.5mg/mL and the average recovery was 99.22%. Conclusion: This method is simple, effective and suitable for analysis of the gliotoxin. A reliable basis was provided for the determination of gliotoxin.

You might also be interested in these eBooks

Info:

Periodical:

Advanced Materials Research (Volumes 581-582)

Pages:

46-49

Citation:

Online since:

October 2012

Export:

Price:

Permissions CCC:

Permissions PLS:

Сopyright:

© 2012 Trans Tech Publications Ltd. All Rights Reserved

Share:

Citation:

[1] J.R. Johnson, W.F. Bruce, and J.D. Dutcher, Gliotoxin, the antibiotic principle of Gliocladium fimbriatum. I. Production, physical and biological properties, J. Am. Chem. Soc. Vol. 65(1943), pp.2005-2009.

DOI: 10.1021/ja01250a051

Google Scholar

[2] W.F. Bruce J.D. Dutcher J.R. Johnson,and L.L. Miller, Gliotoxin, the antibiotic principle of Gliocladium fimbriatum. II. General chemical behavior and crystalline derivatives, American Chemical Society, Vol. 66(1944), p.614–616.

DOI: 10.1021/ja01232a031

Google Scholar

[3] J. Fridrichsons,and A.M. Mathieson, The crystal structure of gliotoxin, Acta Crystallogr., Vol. 23(1967), p.439–448.

DOI: 10.1107/s0365110x67002944

Google Scholar

[4] R. Weindling, and O.H. Emerson, The isolation of a toxic substance from the culture filtrate of Trichoderma, Phytopath., Vol. 26(1936), pp.1068-1070.

Google Scholar

[5] T.W. Jordan, and S.J. Cordiner, Fungal epipolythiodioxopiperazine toxins have therapeutic potential and roles in disease, Trends in Pharmacological Sciences, Vol. 8(1987),pp.144-149.

DOI: 10.1016/0165-6147(87)90184-2

Google Scholar

[6] H.C. Ottenheijm, J.D. Herscheid, and M.W. Tijhuis,et al, Gliotoxin analogues as inhibitors of reverse transcriptase.1.Effect of lipophilicity, Journal of Medical Chemistry,Vol. 21(1978), pp.796-799.

DOI: 10.1021/jm00206a015

Google Scholar

[7] P.L. Rodriguez, and L. Carrasco, Gliotoxin: inhibitor of poliovirus RNA synthesis that blocks the viral RNA polymerase 3Dpol, Journal of Virology, Vol. 66(1992), pp.1971-1977.

DOI: 10.1128/jvi.66.4.1971-1976.1992

Google Scholar

[8] A. Mullbacher, P. Waring, and R.D. Eichner, Identification of an phagocytic and immunomodulating activityin vitro, Journal of General Microbiology, Vol. 131(1985) , pp.1251-1258.

DOI: 10.1099/00221287-131-5-1251

Google Scholar

[9] R.D. Eichner, M. Salami, P.R. Wood, et al, The effect of gliotoxin upon macrophage function, International Immunopharmacology, Vol. 8(1986), pp.789-797.

DOI: 10.1016/0192-0561(86)90016-0

Google Scholar

[10] P. Waring, R.D. Eichner, and A.Mullbacher, et a, Gliotoxin induces apoptosis in macrophages unrelated to its antiphagocytic properties, Journal of Biological Chemistry, Vol. 263(1988), pp.18493-18499.

DOI: 10.1016/s0021-9258(19)81385-6

Google Scholar

[11] P. Waring, DNA fragmentation induced in macrophages by gliotoxin does not require protein synthesis and is preceded by raised inositol triphosphate levels, Journal of Biological Chemistry, Vol. 265(1990), pp.14476-14480.

DOI: 10.1016/s0021-9258(18)77327-4

Google Scholar

[12] J.R. Lissing, B.E. Tuch, and M.G. Suranyi, The use of gliotoxin in human fetal pancreas transplantation, Transplantation Proceedings, Vol. 20(1988), pp.76-78.

Google Scholar

[13] M. Kobayashi, A. Mullbacher, and P. Waring, et al, Gliotoxin treatment selectively spares M-CSF-plus IL-3-responsive multipotent haemopoietic progenitor cells in bone marrow, Eur. J. Haematol., Vol. 46(1991), pp.205-211.

DOI: 10.1111/j.1600-0609.1991.tb00542.x

Google Scholar

[14] P.C. McMinn, G.M. Halliday, and H.K. Muller, Effects of gliotoxin on langerhans cell function: contact hypersensitivity responses and skin graft survival, Immunology, Vol. 71(1990), pp.46-51.

Google Scholar

[15] P.C. McMinn, G.M. Holliday, and P. Waring, et a,l, Langerhans cell depletion in gliotoxin-treated murine epidermis, Pathology, Vol. 23(1991), pp.39-44.

DOI: 10.3109/00313029109061439

Google Scholar

[16] J.E. Karp, and S. Broder, New directions in molecular medicine, Cancer Res., Vol. 54(1994), pp.653-665.

Google Scholar