Determination of Paeoniflorin and Paeonol in Houyinan Tablet by UPLC

Chu Qin Yu; Hua Qing Lin; Yue Han Hou; Zhong Feng Shi; Di Shi Lin

doi:10.4028/www.scientific.net/AMR.581-582.68

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HomeAdvanced Materials ResearchAdvanced Materials Research Vols. 581-582Determination of Paeoniflorin and Paeonol in...

Determination of Paeoniflorin and Paeonol in Houyinan Tablet by UPLC

Abstract:

In this study, our purpose was to establish a UPLC method for the simultaneous determination of Paeoniflorin and Paeonol in Houyinan Tablet. The separation was performed on Acquity BEH C18 column(2.1mm×100mm,1.7μm), the mobile phase was acetonitrile-water with gradient elution at a flow rate of 0.2 mL•min-1, the detection wavelength was 230nm, the column temperature was 30°Cand the injection volume was 2μL. Paeoniflorin and Paeonol reached effective separation with the other components in this chromatographic conditions. Paeoniflorin and Paeonol were linear within the range of 0.0406~0.4064μg(r=0.9999) and 0.0426~0.4256μg (r=0.9999), respectively. The average recovery was 99.82% and 100.6%. The results of method validation indicated that the method was simple,quick,accurate, specific and less solvent consumption. It can be used for the quality control of Houyinan Tablet.

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Periodical:

Advanced Materials Research (Volumes 581-582)

Pages:

68-72

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.581-582.68

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Online since:

October 2012

Authors:

Chu Qin Yu, Hua Qing Lin, Yue Han Hou, Zhong Feng Shi, Di Shi Lin

Keywords:

Determination, Houyinan Tablet, Paeoniflorin, Paeonol, UPLC

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References

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