Enzymatic Production of γ-Aminobutyric Acid Using Rice Bran

Abstract:

An enzymatic method for γ-aminobutyric acid (GABA) production was invested. With this method, rice bran was used as glutamate decarboxylase (GAD) source and exogenous monosodium glutamate(MSG) was used as substrate. We stimulated the rice bran GAD via regulating the temperature, pH, reaction time, buffer and adding PLP, Ca2+ and substrate. In the existence of PLP and Ca2+, the GABA content of rice bran had been improved by about 45 fold. The GABA production reached 2.3g/100g bran, and the Glu conversion reached 100%. As rice bran is a by-product in rice processing and a large quantity of rice bran is commercially available, our study illuminated a safe and efficient way to produce GABA and GABA enriched food.

• Periodical: Advanced Materials Research (Volume 586)
• Pages: 85-91
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.586.85
• Online since: November 2012
• Authors: Ying Guo Lü, Hui Zhang, Hui Yuan Yao
• Keywords: Enzymatic Production, Glutamate Decarboxylase, Monosodium Glutamate, Rice Bran, γ-Aminobutyric Acid (GABA)

References

[1] Mody, I., Dekoninck, Y., Otis, T. S., Scoltesz, I. Bring the cleft at GABA synapses in the brain. Trends Neurosci. (1994) 17: 517-525.

DOI: 10.1016/0166-2236(94)90155-4

[2] Manyam, B. V., Katz, L., Hare, T. A., Kanifefski, K., Tremblay, R. D. Isoniazid-induced elevation of cerebrospinal fluid (CSF) GABA levels and effects on chorea in Hunitington's disease. Annu. Neurol. (1981)10: 35–37.

DOI: 10.1002/ana.410100107

[3] Jakobs, C., Jaeken, J., Gibson, K., M. Inherited disorders of GABA metabolism. J. Inher. Met. Dis. (1993)16(4): 704–715.

DOI: 10.1007/bf00711902

[4] Takahashi, H., Sumi, M., and Koshino, F. Effect of γ-aminobutyric acid (GABA) on normotensive or hypertensive rats and men. Jpn. J. Physiol (1961) 11: 89-95.

DOI: 10.2170/jjphysiol.11.89

[5] Omori, M., Yano, T., Okamoto, J., Tsushida, T., Murai, T., Higuchi, M. Effect of anaerobically treated tea (gabaron tea) on blood pressure of spontaneously hypertensive rats. J. Jpn. Soc. Biosci. Biotech. (1987)61(11): 1449–1451.

DOI: 10.1271/nogeikagaku1924.61.1449

[6] Okada, T., Sugishita, T., Murakami, T., Murai, H., Saikusa, T., Horio, T., Onoda, A., Kajimoto, O., Takahashi, R., Takahashi, T. Effect of the defatted rice germ enriched with GABA for sleepless, depression, autonomic Disorder by oral administration. J. Jpn. Soc. Food Sci. Tech. (2000).

DOI: 10.3136/nskkk.47.596

[7] Sawai, Y., Konomi, K., Odaka, Y., Yoshitomi, H., Yamaguchi, Y., Miyama, D., et al. Repeating treatment of anaerobic and aerobic incubation increases the amount of γ-aminobutyric acid in tea shoots. J. Jpn. Soc. Food Sci. Tech. (1999).

DOI: 10.3136/nskkk.46.462

[8] William Wallace, Jacob Secor, Larry E. Schrader. Rapid Accumulation of γ-Aminobutyric Acid and Alanine in Soybean Leaves in Response to an Abrupt Transfer to Lower Temperature, Darkness, or Mechanical Manipulation. Plant Physiol (1984).

DOI: 10.1104/pp.75.1.170

[9] Takayo Saikusa, Toshiroh Horino, Yutaka Mori. Accumulation of γ-Aminobutyric Acid (Gaba) in the rice germ during water soaking. Biosci. Biotech . Biochem. (1994) 58(12): 2291-2292.

DOI: 10.1271/bbb.58.2291

[10] Kinefuchi, M., Sekiya, M., Yamazaki, A., and Yamamoto, K. Accumulation of GABA in brown rice by high pressure treatment. J. Jpn. Soc. Food Sci. Tech. (1999) 46(5): 323–328.

[11] Noriko Komatsuzaki, Kikuichi Tsukahara, Hidechika Toyoshima, Tadanao Suzuki, Naoto Shimizu, Toshinori Kimura. Effect of soaking and gaseous treatment on GABA content in germinated brown rice. J. Food Eng. (2007)78: 556–560.

DOI: 10.1016/j.jfoodeng.2005.10.036

[12] Takayo Saikusa, Toshiroh Horino, Yutaka Mori. Distribution of Free Amino Acid in the Rice Kernal and Kernal Fraction and the Effect of Water soaking on the Distribution. J. Agric. Food Chem. (1994)42: 1122-1125.

DOI: 10.1021/jf00041a015

[13] Liu, L.L., Zhai, H.Q., Wan, J. -M. Accumulation of γ-aminobutyric acid in giant-embryo rice grain in relation to glutamate decarboxylase activity and its gene expression during water soaking. Cereal Chem. (2005)82(2): 191-196.

DOI: 10.1094/cc-82-0191

[14] Ohtsubo, S., Asano, S., Sato, K., Matsumoto, I. Enzymatic Production of γ-Aminobutyric Acid Using Rice (Oryza sativa) Germ. Food Sci. Tech. Res. (2000)6(3): 208-211.

DOI: 10.3136/fstr.6.208

[15] Zhang, H., Yao, H. Y., Chen, F., Accumulation of γ-aminobutyric acid in rice germ using protease. Biosci. Biotech. Biochem. (2006)70(5): 1160-1165.

DOI: 10.1271/bbb.70.1160

[16] Ito Gong. Production of GABA-enriched rice bran. Food Ind. (2000)43(10): 61-64.

[17] Zhang, H., Yao, H. Y., Chen, F., Wang, X., Purification and characterization of glutamate decarboxylase from rice germ. Food Chem. (2007)101(4): 1670-1676.

DOI: 10.1016/j.foodchem.2006.04.027

[18] Satya Narayan V., Nair P. M. Metabolism, Enzymology and possible roles of 4-aminobutyrate in higher plants. Phytochem. (1990)29: 367-375.

DOI: 10.1016/0031-9422(90)85081-p

[19] Martin, D. L., Rimvall, K. Regulation of gamma-aminobutyric acid synthesis in the brain. J. Neurochem. (1993)60: 395–407.

DOI: 10.1111/j.1471-4159.1993.tb03165.x

[20] Baum, G., Chen, Y., Arazi, T., Takatsuji, H., Fromm, H. A plant glutamate decarboxylase containing a calmodulin binding domain. Cloning, sequence, and functional analysis. J. Bio. Chem. (1993)68: 19610–19617.

DOI: 10.1016/s0021-9258(19)36560-3

[21] Snedden, W. A., Koutsia, N., Baum, G., Fromm, H. Activation of a recombinant petunia glutamate decarboxylase by calcium/calmodulin or by a monoclonal antibody which recognizes the calmodulin binding domain. J. Bio. Chem. (1996)271: 4148–4153.

DOI: 10.1074/jbc.271.8.4148

[22] Snedden, W. A., Arazi, T., Fromm, H., Shelp, B. J. Calcium/calmodulin activation of soybean glutamate decarboxylase. Plant Physiol. (1995)108: 543–549.

DOI: 10.1104/pp.108.2.543

[23] Johnson, B. S., Singh, N. K., Cherry, J. H., Locy, R. D. Purification and characterization of glutamate decarboxylase from cowpea. Phytochem. (1997)46(1): 39–44.

DOI: 10.1016/s0031-9422(97)00236-7

[24] Aurisano, N., Bertani, A., Reggiani, R. Anaerobic accumulation of 4-aminobutyrate in rice seedlings; causes and significance. Phytochem. (1995)38: 1147–1150.

DOI: 10.1016/0031-9422(94)00774-n

[25] Barry J. Shelp, Alan W. Bown , Michael D. McLean. Metabolism and functions of gamma-aminobutyric acid. Trends plant sci. (1999)4(11): 446-452.

DOI: 10.1016/s1360-1385(99)01486-7

[26] Snedden, W.A., Fromm, H. Calmodulin, calmodulin-related proteins and plant responses to the environment. Trends Plant Sci. (1998)3: 299-304.

DOI: 10.1016/s1360-1385(98)01284-9

[27] Hayakawa, K., Kimura, M., Kasaha, K., Matsumoto, K., Sansawa, H., Yamori, Y. Effect of a gamma-aminobutyric acid-enriched dairy product on the blood pressure of spontaneously hypertensive and normotensive Wistar-Kyoto rats. Br. J. Nutr (2004).

DOI: 10.1079/bjn20041221

[28] Ki-Bum Park, Suk-Heung Oh. Production of yogurt with enhanced levels of gamma-aminobutyric acid and valuable nutrients using lactic acid bacteria and germinated soybean extract. Bioresour. Technol. (2007)98: 1675–1679.

DOI: 10.1016/j.biortech.2006.06.006