Activity of Deinococcus radiodurans on Chromium Ions

Abstract:

This study investigated the resistance of Deinococcus radiodurans to Cr6+ and Cr3+, the redox activity of this bacterium for Cr6+ and Cr3+ and the contributions of various bacterial components to the observed chromium resistance. The results showed that at 0.2 and 0.5 mmol/L of Cr6+, D. radiodurans displayed high growth with an early logarithmic phase. This organism also exhibited moderate growth at 2 mmol/L Cr3+. This strain exhibited strong reduction activity against Cr6+. At 0.2 mmol/L Cr6+, the 8-hour Cr6+ clearance rate was 92.13%. However, the clearance rate decreased sharply with increasing concentrations of Cr6+. At 0.5 mmol/L Cr6+, the 24-hour clearance rate was only 32.81%. By studying the effects of the cellular environment and individual bacterial components on the level of Cr6+, we discovered that D. radiodurans counteracted the effects of Cr6+ primarily through reduction and secondarily through adsorption. The primary activity was found to occur in the extracellular space and the cell culture supernatant. D. radiodurans had no significant oxidation effects on Cr3+. In conclusion, this study showed that D. radiodurans strongly reduces and is highly tolerant of Cr6+ and that the primary reducing agent is secreted from the cell.

• Periodical: Advanced Materials Research (Volume 599)
• Pages: 118-123
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.599.118
• Online since: November 2012
• Authors: Yun Xiao He, Xiao Ming Chen
• Keywords: Heavy Metal Pollution, Hexavalent Chromium, Microorganisms Redox, Trivalent Chromium, Valence State

References

[1] M. Megharaj, S.Avudainayagam, and R. Naidu: Current Microbiology vol.47(2003), p.51

[2] M.Faisal, A.Hameed, and S. Hansnain: Journal of Industrial Microbiology ＆ Biotechnology vol.32(2005), p.615

[3] T.T. Srinath, and P.W. Ramtake: Chemiosphere vol.48(2002) , p.427

[4] S.Sultan, and S.Hansnain: Bulletin of Environmental Contamination ＆ Toxicology vol.75(2005) , p.699

[5] Vieram, G. Curutchet, and Donatie: Biodeterioration ＆ Biodegeradation vol.52(2003), p.31

[6] M.T. Bradley, and A.Y. Obraztsova: FEMS Microbiology Letters vol.162(1998),p.193

[7] Y.J. Feng: Environmental Pollution & Control vol.17(1995),p.15

[8] K.S. Makarova, L.Aravind, and Y.I. Wolf: Microbiol Mol Biol Rev. vol.65(2001), p.44

[9] J. D.Michael: Nature Reviews Microbiology vol.7(2009), p.237

[10] R.N. Horton, W.A. Apel, and V.S. Thompson: B M C Microbiology vol.6(2006), p.5

[11] W.Xiao: Industrial Microbiology vol.37(2007),p.1

[12] L.Philip, L.Iyengar, and C. Venkobachar: J Envir Engrg vol.124(1998),p.1165

[13] C.Garbisu, I.Alkorta, and M.J. Llama: Biodegradation vol.9(1998) ,p.133

[14] D.R. Lovely,and E.J.P. Phillips: Appl Environ Microbiol 60(1994),p.722

[15] Z.Aksu, and D.Akpinar: Biochemical Engineering Journal vol.7(2001),p.183

[16] P. Pattanapipitpaisal, A.N. Mabbett, and J.A. Finlay: Environmental Technology 2002; vol.23(2002),p.731

[17] U.Thacker, and D. Madamwar: World Journal of Microbiology ＆ Biotechnology 2005 vol.21(2005),p.891