Screening of a Thermophilic Hydrocarbon-Degrading Bacterial Consortium and Community Structure Analysis

Abstract:

5 highly efficient hydrocarbon-degrading mixed bacteria were obtained from the petroleum-contaminated soil samples by the traditional method of enrichment and acclimation. The KL9-1 consortium had a wide temperature tolerance range and higher hydrocarbon degrading ability. The degradation rate of thin oil and heavy oil was up to 43.27% and 20.09% respectively through 7 d at 45^oC. After several times of isolation and purification, three dominant strains with the capability of hydrocarbon-degrading were obtained, and their petroleum degradation rates were all over 30%. According to its morphological and 16S rDNA sequence characteristics, KL9-1-1 and KL9-1-2 were identified as Pseudomonas sp., and the KL9-1-3 was identified as Rhodococcus sp..

• Periodical: Advanced Materials Research (Volumes 610-613)
• Pages: 225-228
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.610-613.225
• Online since: December 2012
• Authors: Qi You Liu, Lin Li, Dong Feng Zhao, Chao Cheng Zhao
• Keywords: 16S rDNA, Bioremediation, Petroleum Polluted Soil, Screening

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