Matrine Determination in Compound Sophora Alopecuroides Suppositories by High-Performance Liquid Chromatography

Abstract:

The determination of matrine in compound sophora alopecuroides suppositories was carried out by reversed-phase high-performance liquid chromatography(RP-HPLC). Elite ODS C18(250mm×4.5mm, 5µm) column was used and acetonitrile -anhydrous ethanol-water-triethy lamine (15:22.5:62.5:0.01) was selected as the mobile phase. Meanwhile the detection wavelength 215nm was used. The linearity of matrine was in the range of 0.74-5.18µg，r=0.9991，and the average recovery was 98.52%，RSD was 1.54%. The method was proved to be simple, accurate, sensitive and reliable. It can be used for the quality control of compound sophora alopecuroides suppositories.

• Periodical: Advanced Materials Research (Volumes 634-638)
• Pages: 1001-1004
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.634-638.1001
• Online since: January 2013
• Authors: Hui Xiang Hu, Xuan Zhang, Zhimin Zhang
• Keywords: Compound Sophora Alopecuroides Suppositories, Matrine, RP-HPLC

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