Studies on Transformation of Alfalfa with Chicken β-Defensin Gal-3

Abstract:

Chicken β-defensin Gal-3 was recently identified member of a family of antimicrobial peptides. In this paper, the sequences of the chicken β-defensin Gal-3 gene was replaced with plant preferred codons by Polymerase Chain Reaction, and cloned into the plant expression vector p1390R, then transferred into alfalfa by Agrobacterium tumefacien- mediated method. PCR was performed by using the genomic DNA of transgenic alfalfa as template, which showed that Gal-3 has been transferred into alfalfa. Therefore, the suitable concentration of Hyg for screening transformants was 20 mg/L. The 4 trangenic plants were analyzed by Southern hybridization.

• Periodical: Advanced Materials Research (Volumes 634-638)
• Pages: 1366-1370
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.634-638.1366
• Online since: January 2013
• Authors: Li Bo Jin, Xiu Ming Liu, Yong Jiang, Lei Liu, Shu Huan Du, Hui Yan Wang
• Keywords: Agrobacterium tumefacien, Alfalfa, Transgenic Plants, β-Defensin Gal-3

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DOI: 10.1016/s1387-2656(07)13006-4