Applications of Ultrafiltration-Nanofiltration Membrane Continuous Combination Technology for Refining of Milk-Derived Oligosaccharides

Abstract:

The galactooligosaccharide (GOS) was produced by Bulgarian lactic acid bacteria fermentation and further refined by application of an ultrafiltration-nanofiltration continuous membrane combination technology established in this paper. Results revealed that the optimum of membrane operation conditions for ultrafiltration were 0.05 Mpa at the feed concentration of 2.5 g/L, and the optimal conditions for nanofiltration were 45 °C, 0.4 Mpa. After refining by membrane combination technology, 96.70% of proteins were removed and the relative quality ratio of the refined GOS product was increased by 22 times or so, which mainly contained oligomers with DP 3 and DP 2, and mostly distributed between 108 Da and 2415 Da. These findings suggest that the ultrafiltration-nanofiltration continuous membrane combination technology is a new and favorable processes method for enrich or refining of milk-derived GOS obtained by fermentation.

• Periodical: Advanced Materials Research (Volumes 634-638)
• Pages: 1429-1434
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.634-638.1429
• Online since: January 2013
• Authors: Sheng Ming An, Jin Hong Wu, Li Fang Qian, Yan Ling Gao, Yan Wu, Guo Ping Yu
• Keywords: Degree of Polymerization, Galactooligosaccharide, Nanofiltration, Refining, Ultrafiltration (UF)

References

[1] P. Zheng, H. Yu, Z. Sun, Y. Ni, W. Zhang, Y. Fan and Y. Xu, Production of galacto-oligosaccharides by immobilized recombinant β-galactosidase from Aspergillus candidus, Biotechnol J. 1 (2006) 1464-1470.

DOI: 10.1002/biot.200600100

[2] N. Onishi, A. Yamashior, K. Yokozeki, Production of Galacto-Oligosaccharide from Lactose by Sterigmatomyces elviae CBS8119, Applend and environmental microbiology. 11 (1995) 4022-4025.

DOI: 10.1128/aem.61.11.4022-4025.1995

[3] R.E. Ward, Isolation of Milk Oligosaccharides using Solid-Phase Extraction, Open Glycoscience. 2 (2009) 9-15.

DOI: 10.2174/1875398100902010009

[4] D.B. Sarney, C. Hale, G. Frankel and E.N. Vulfson, A novel approach to recovery of biologically active oligosaccharides from milk using a combination of enzymatic treatment and nanofiltrstion, Biotech Bioeng, 69 (2000) 461-467.

DOI: 10.1002/1097-0290(20000820)69:4<461::aid-bit13>3.0.co;2-l

[5] J. Gyura, Z. Seres, and M. Eszterle, Influence of operating parameters on separation of green syrup colored matter from sugar beet by ultra- and nanofiltration, J Food Eng. 66 (2005) 89-96.

DOI: 10.1016/j.jfoodeng.2004.02.038

[6] A. Geisser, T. Hendrich, G. Boehm, and B. Stahl, Separation of lactose from human milk oligosaccharides with simulated moving bed chromatography, J Chrom. 1092 (2005) 17-23.

DOI: 10.1016/j.chroma.2005.03.061

[7] Determination of trans-Galactooligosaccharides (TGOS) in Selected Food Products, AOAC Official Method. (2001.02).

[8] O.H. Lowry, N.J. Rosebrough, A.L. Farr, and R.J. Randall, J Biol Chem. 193 (1951) 265.

[9] G.L. Miller, Use of dinitrosalicylic acid reagent for determination of reducing sugar, Analytical Chemistry. 31 (1959) 426-428.

DOI: 10.1021/ac60147a030

[10] S.J. Ma, Y. Zhang, C. Zhao, X.Y. and H.F. Qian, Separation and properties of polysaccharides from asparagus (Officinalis L) old stalks, Advanced Materials Research. (2012) 361-363.

DOI: 10.4028/www.scientific.net/amr.361-363.789

[11] T. Tsuru, S. Izumi, and T. Yoshioka, Temperature effect on transport performance by inorganic nanofiltration membranes, AIChE Journal. 46 (2000) 565-574.

DOI: 10.1002/aic.690460315

[12] Y.M. Feng, X.L. Chang, W.H. Wang, and R.Y. Ma, Separation of galacto-oligosaccharides mixture by nanofiltration, Journal of the Taiwan Institute of Chemical Engineers. 40 (2009) 326-332.

DOI: 10.1016/j.jtice.2008.12.003