Antioxidant Properties of Deer Blood Hydrolysate and the Possible Mode of Action

Article Preview

Abstract:

Deer blood was hydrolyzed using Alcalase with hydrolysis time ranged form 0 to 6 h, and the degree of hydrolysis (DH) of protein hydrolysates increased with increasing hydrolysis time (P < 0.05). The reducing power, radicals scavenging activities and Cu2+-chelation ability of deer blood hydrolysate (DBH) significantly enhanced with increasing hydrolysis time (P < 0.05). The antioxidant activity of DBH, indicated by thiobarbituric acid-reactive substance (TBARS) values in a liposome-oxidizing system, increased with increasing DH (P < 0.05). The results indicated that antioxidant activity of DBH depended on hydrolysis time, and the hydrolyzed deer blood could be a potent food antioxidant.

You might also be interested in these eBooks

Info:

Periodical:

Advanced Materials Research (Volumes 634-638)

Pages:

1435-1440

Citation:

Online since:

January 2013

Export:

Price:

Permissions CCC:

Permissions PLS:

Сopyright:

© 2013 Trans Tech Publications Ltd. All Rights Reserved

Share:

Citation:

[1] C. Y. Chang, K. C. Wu, and S. H. Chiang, Antioxidant properties and protein compositions of porcine haemoglobin hydrolysates, Food Chem. 100 (2007) 1537–1543.

DOI: 10.1016/j.foodchem.2005.12.019

Google Scholar

[2] C. B. Ahn, Y. J. Jeon, D. S. Kang, D. S. Shin, and B. M. Jung, Free radical scavenging activity of enzymatic extracts from a brown seaweed Scytosiphon lomentaria by electron spin resonance spectrometry, Food Res. Int. 37 (2004) 253–258.

DOI: 10.1016/j.foodres.2003.12.002

Google Scholar

[3] A. Wanita, and K. Lorenz, Antioxidant potential of 5-Npentadecylresorcinol, J. Food Process Pres. 20 (1996) 417–429.

Google Scholar

[4] N. S. Hettiarachchy, K. C. Glenn, R. Gnanasambandam, and M. G. Johnson, Natural antioxidant extract from fenugreek (Trigonella foenumgraecum) for ground beef patties, J. Food Sci. 61 (1996) 516–519.

DOI: 10.1111/j.1365-2621.1996.tb13146.x

Google Scholar

[5] E. A. Peña-Ramos, and Y. L. Xiong, Antioxidative activity of whey protein hydrolysates in a liposomal system, J. Dairy Sci. 84 (2001) 2577–2583.

DOI: 10.3168/jds.s0022-0302(01)74711-x

Google Scholar

[6] C. M. Liyana-Pathiranaa, and F. Shahidi, Antioxidant and free radical scavenging activities of whole wheat and milling fractions, Food Chem. 101 (2007) 1151–1157.

DOI: 10.1016/j.foodchem.2006.03.016

Google Scholar

[7] B. H. Kong, and Y. L. Xiong, Antioxidant activity of zein hydrolysates in a liposome system and the possible mode of action, J. Agr. Food Chem. 54 (2006) 6059–6068.

DOI: 10.1021/jf060632q

Google Scholar

[8] J. Adler-Nissen, Enzymic hydrolysis of food proteins, New York, USA: Elsevier Applied Science Publishers, 1986.

Google Scholar

[9] E. A. Decker, and H. O. Hultin, Factors influencing catalysis of lipid oxidation by the soluble fraction of mackerel muscle, J. Food Sci. 55 (1990) 947–950, 953.

DOI: 10.1111/j.1365-2621.1990.tb01571.x

Google Scholar

[10] R. Amarowicz, Antioxidant activity of protein hydrolysates, Eur. J. Lipid Sci. Tech. 110 (2008) 489–490.

DOI: 10.1002/ejlt.200800120

Google Scholar

[11] Q. Liu, B. H. Kong, L. Z. Jiang, X. H. Cui, and J. Liu, Free radical scavenging activity of porcine plasma protein hydrolysates determined by electron spin resonance spectrometer, LWT-Food Sci. Technol. 42 (2009) 956–962.

DOI: 10.1016/j.lwt.2008.12.007

Google Scholar

[12] Q. Liu, B. H. Kong, Y. L. Xiong, and X. F. Xia, Antioxidant activity and functional properties of porcine plasma protein as influenced by the degree of hydrolysis, Food Chem. 118 (2010) 403–410.

DOI: 10.1016/j.foodchem.2009.05.013

Google Scholar

[13] S. Y. Jun, P. J. Park, W. K. Jung, and S. K. Kim, Purification and characterization of an antioxidative peptide from enzymatic hydrolysate of yellowfin sole (Limanda aspera) frame protein, Eur. Food Res. Tech. 219 (2004) 20–26.

DOI: 10.1007/s00217-004-0882-9

Google Scholar

[14] H. C. Wu, H. M. Chen, and C. Y. Shiau, Free amino acids and peptides as related to antioxidant properties in protein hydrolysates of mackerel (Scomber austriasicus), Food Res. Int. 36 (2003) 949–957.

DOI: 10.1016/s0963-9969(03)00104-2

Google Scholar