Discrimination and Simultaneous Determination of 6-BA and KT by Polarized Synchronous Light Scattering and Synchronous Light Scattering Spectrum

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Abstract:

In pH=5.70 B-R buffer solution, Na2WO4 and rhodamine 6G(R6G) could react with natural cytokinins of 6-benzylaminourine (6-BA) and kinetin (KT) and resulted in a great enhacement of synchronous light scattering (SLS) signals. This article first established the degree of polarized synchronous light scattering (P) based on the measurements of the polarized synchronous light scattering signals to distinguish two natural cytokinins of 6-BA and KT. Simultaneously based on the synchronous scattering spectrum and the double standard curve method, a novel method for simultaneous determination of 6-BA and KT was developed by SLS spectralmethod. The method was applied to simultaneous determination of 6-BA and KT in balsam pear skin and tomato skin with satisfactory results.

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Periodical:

Advanced Materials Research (Volumes 634-638)

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81-86

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January 2013

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© 2013 Trans Tech Publications Ltd. All Rights Reserved

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