Characterization of Codon Usage Bias in the UL17 Gene of Duck Enteritis Virus

Abstract:

The analysis on codon usage bias of UL17 gene of duck enteritis virus (DEV) may provide a basis for understanding the relevant mechanism for its biased usage of synonymous codons and for selecting appropriate host expression systems to improve the expression of DEV UL17 gene. In this study the results indicate that codon usage bias of DEV UL17 gene strongly preferred to the synonymous with A and T at third codon position. The ENC values and GC3S contents of the codon usage bias of UL17 genes in DEV and the 20 reference herpesviruses were obviously different. In addition, we compared the codon usage bias of DEV UL17 gene with E.coli, yeast and human. There are 25 codons showing distinct usage differences between DEV and E. coli, 17 codons between DEV and yeast, 23 codons between DEV and human. Therefore, the yeast expression system is more suitable for heterologous expression of the DEV UL17 gene.

• Periodical: Advanced Materials Research (Volumes 641-642)
• Pages: 666-674
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.641-642.666
• Online since: January 2013
• Authors: Ting Wen, An Chun Cheng, Ming Shu Wang
• Keywords: Codon Usage Bias, Duck Enteritis Virus (DEV), UL17 Gene

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