Influence of Blocking Agents on Non-Specific Background of Polystyrene Microbeads in Serum Immunoassay

Abstract:

In this paper, we used bovine serum albumin and polymer as the blocking agents and investigated the effect of blocking agents on non-specific background of polystyrene microbead that used the human serum immunoassay.The results showed that the nonspecific background is lower by using polymer blocking agents. The best blocking condition was that microbeads were blocked by PVXT (0.5% polyvinyl alcohol PVA, 0.8% polyvinylpyrrolidone, 0.05% Tween-20, PBS phosphate buffer, pH7.0) for two hours at room temperature.

• Periodical: Advanced Materials Research (Volumes 641-642)
• Pages: 858-861
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.641-642.858
• Online since: January 2013
• Authors: Zhi Min Ren, Xi Nie, Sheng Shu Ai
• Keywords: Bovine Serum Albumin (BSA), Poly(vinyl alcohol), Polystyrene Microbead, Polyvinylpyrrolidone

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