Tumstatin 7 Peptide Affect Biological Activity of B16 Melanoma Cell

Shu Jing Wang; Jia Liu; Fei Wang; Ning Chen; Shan Jiang; Lin Liu; Ying Zhao; Xiao Dan Zhang

doi:10.4028/www.scientific.net/AMR.641-642.915

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HomeAdvanced Materials ResearchAdvanced Materials Research Vols. 641-642Tumstatin 7 Peptide Affect Biological Activity of...

Tumstatin 7 Peptide Affect Biological Activity of B16 Melanoma Cell

Abstract:

To study the effect of biological activity of Tumstatin 7 peptide on the cell proliferation and apoptosis of B16 melanoma. Tumstatin 7 peptide was synthesized and its purity is 98.4532% by high-performance liquid chromatography. The effect of 7 peptide on B16 melanoma was observed by MTT, cell growth curve, the observation of the transmission electron microscope (TEM). 7 peptide can significantly inhibit B16 melanoma cell proliferation, showing dose- and time-dependent .Its IC50 was 72.53 μg/ml.The morphology of B16 melanoma cell was obviously changed by TEM, such as karyopyknosis and apoptotic bodies. So7 peptides can inhibit the proliferation of melanoma cells and promote melanoma cells apoptosis. It will be of great potential value to melanoma treatment.

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Periodical:

Advanced Materials Research (Volumes 641-642)

Pages:

915-918

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.641-642.915

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Online since:

January 2013

Authors:

Shu Jing Wang, Jia Liu, Fei Wang, Ning Chen, Shan Jiang, Lin Liu, Ying Zhao, Xiao Dan Zhang

Keywords:

Cell Apoptosis, Cell Proliferation, Melanoma, Tumstatin

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References

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