Characterization and Functional Expression of Xylose Isomerase from Thermus thermophilus

An Gen Lu; Ze Xi Yang; Fei Wang; Lang Xu; Wen Ying Deng; Jian Zong Meng; Zi Qi Huang; Xiang Hui Qi

doi:10.4028/www.scientific.net/AMR.641-642.919

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HomeAdvanced Materials ResearchAdvanced Materials Research Vols. 641-642Characterization and Functional Expression of...

Characterization and Functional Expression of Xylose Isomerase from Thermus thermophilus

Abstract:

Ethanol produced from hexose and pentose sugars hydrolysated by lignocellulose is an environment-friendly alternative to fossil fuels. Xylose isomerase is the major rate-limiting enzyme in the ethanol synthesis biologically pathway of xylose fermentation. In present study, xylA gene encoding xylose isomerase was cloned from Thermus thermophilus and overexpressed in E. coli BL21. Purified recombinant enzyme was used to study the enzymatic characterization. Specific activity of recombinant PDOR was 19.6 U/mg. Optimal temperature and pH were 80 °C, 8.0, respectively. Km and Vmax values were 15.9 mM, 22.8 U/mg. This research may form a basis for the future application of xylose isomerase.

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Periodical:

Advanced Materials Research (Volumes 641-642)

Pages:

919-922

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.641-642.919

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Online since:

January 2013

Authors:

An Gen Lu, Ze Xi Yang, Fei Wang, Lang Xu, Wen Ying Deng, Jian Zong Meng, Zi Qi Huang, Xiang Hui Qi

Keywords:

Characterization, Overexpression, Thermus thermophilus, xylA

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References

[1] Hasunuma T., Sanda T., Yamada R., et al. Metabolic pathway engineering based on metabolomics confers acetic and formic acid tolerance to a recombinant xylose-fermenting strain of Saccharomyces cerevisiae, Microb. Cell Fact. 10 (2011) 2.

DOI: 10.1186/1475-2859-10-2

Google Scholar

[2] Brat D., Boles E., Wiedemann B. Functional expression of a bacterial xylose isomerase in Saccharomyces cerevisiae. Appl. Environ. Microbiol. 75 (2009) 2304-2311.

DOI: 10.1128/aem.02522-08

Google Scholar

[3] Dekker K., Yamagata H., Sakaguchi K., et al. Xylose (glucose) isomerase gene from the thermophile Thermus thermophilus: cloning, sequencing, and comparison with other thermostable xylose isomerases, J. Bacteriol. 173 (1991) 3078-3083.

DOI: 10.1128/jb.173.10.3078-3083.1991

Google Scholar

[4] Umemoto Y., Shibata T., Araki T. D-Xylose Isomerase from a Marine Bacterium, Vibrio sp. Strain XY-214, and D-Xylulose Production from beta-1, 3-Xylan, Mar. Biotechnol. 14(2012): 10-20.

DOI: 10.1007/s10126-011-9380-9

Google Scholar

[5] Ravikumar S., Vikramathithan J., Srikumar K. Purification and characterization of a novel thermostable xylose isomerase from Opuntia vulgaris mill, Appl. Biochem. Biotechnol. 164 (2011) 593-603.

DOI: 10.1007/s12010-011-9160-z

Google Scholar

[6] Ravikumar S., Shyamala S., Muthuraman P., et al. Isolation, purification, and characterization of thermophilic T80 isoenzyme of xylose isomerase from the xerophyte Cereus pterogonus, Prep. Biochem. Biotechnol. 41(2011)122-137.

DOI: 10.1080/10826068.2011.547342

Google Scholar

[7] Dekker K., Sugiura A., Yamagata H., et al. Efficient production of thermostable Thermus thermophilus xylose isomerase in Escherichia coli and Bacillus brevis, Appl. Microbiol. Biotechnol. 36 (1992) 727-732.

DOI: 10.1007/bf00172183

Google Scholar