A Surface-Enhanced Resonance Raman Scattering Method for Trace Cystine Using 4-Mercaptopyridine-Aggregated-Nanosilver as Probe

Yan Yan Wei; Fang Li; Ai Hui Liang; Zhi Liang Jiang

doi:10.4028/www.scientific.net/AMR.647.181

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A Surface-Enhanced Resonance Raman Scattering Method for Trace Cystine Using 4-Mercaptopyridine-Aggregated-Nanosilver as Probe
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HomeAdvanced Materials ResearchAdvanced Materials Research Vol. 647A Surface-Enhanced Resonance Raman Scattering...

A Surface-Enhanced Resonance Raman Scattering Method for Trace Cystine Using 4-Mercaptopyridine-Aggregated-Nanosilver as Probe

Abstract:

A stable nanosilver solution was prepared, using PEG10000 as stabilizer and NaBH4 as reducer. In pH 6.6 Na2HPO4-NaH2PO4 buffer solution containing PEG10000 and NaCl, nanosilvers were aggregated to form the stable aggregated-nanosilvers (ANS) that could conjugate with 4-mercaptopyridine (MP) to produce an ANS-MP surface-enhanced resonance Raman scattering (SERRS) probe, which exhibited a strong SERS peak at 1097 cm-1. When cystine (Cy) concentration increased, the SERS intensity at 1097 cm-1 decreased linearly. In the optimal condition, the decreased SERRS intensity was linear to Cy concentration in the range of 5.0×10-9-1.4×10-7 mol/L, with a detection limit of 2.0×10-9 mol/L Cy.

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Periodical:

Advanced Materials Research (Volume 647)

Pages:

181-184

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.647.181

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Online since:

January 2013

Authors:

Yan Yan Wei, Fang Li, Ai Hui Liang, Zhi Liang Jiang

Keywords:

4-Mercaptopyridine, Aggregated-Nanosilver Suspension, Cystine, Surface-Enhanced Raman Scattering

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