Micro-Lithographic Fabrication of Collagen and Hyaluronic Acid Hydrogel Scaffolds

Abstract:

The regeneration of human diseased parts is based on substantial cell number to support physiological functions of diseased tissues/organs. To this end, the scaffolds for cellular proliferation should be structurally similar to normal tissues. This study aimed to fabricate biomimetic structures of naturally-occurring polymer. We utilized micro-lithography to produce three-dimensional scaffold of the composite of collagen and hyaluronic acid (HA) which is further used to evaluate the cellular attachment and proliferation. Collagen from porcine skin was characterized by SDS-PAGE that three species having molecular weights of 120, 130 and 250 kDa were assigned as 2, 1 and  chains of type I collagen. HA was purified by diafiltration and characterized by carbzaole method. The purity of HA was increased from 41.6 g/mL to 71.9 g/mL. To endow photo-cross linker to HA, a graft reaction, catalyzed by triethyl amine was carried out that glycidyl methacrylate (GM) was conjugated to side group of HA. Chemical shifts of 5.7 and 6.1 ppm of 1H NMR spectrum indicated that GMHA was synthesized. To develop 3D scaffolds, we formulated a weight ratio of 10:3 for GMHA and collagen as precursor of micro-lithography. Photo masks of curved, fine lines and rectangular patterns were utilized. For finest lines (100 m) in the pattern, it took 9 minutes to fully crosslink the formulation with UV light, whereas curved lines of 2 to 5 mm could be cross linked in 2 minutes. The scaffolds were found to have pores of 10- 100 m observed under scanning electron microscope. This study completed the scaffold fabrication of naturally-occurring polymers and confirmed the optimized conditions of micro-lithography. The 3D scaffolds having linear and curved lines and rectangular patterns will be used to evaluate the cellular attachment and migration of various mammalian cells.

• Periodical: Advanced Materials Research (Volume 647)
• Pages: 170-175
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.647.170
• Online since: January 2013
• Authors: Ming Fa Hsieh, Chia Lin Sheu, Hsi Wen Yang
• Keywords: Collagen, Hyaluronic Acid, Photo-Crosslinking, Photo-Lithography, Tissue Engineering

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