Effect of Folin Reagent from Different Sources on Protease Determination

Jun Liang Sun; Xin Hong Liang; Song Ling Chen; Ying Bin Fan

doi:10.4028/www.scientific.net/AMR.662.305

Paper Titles

Preparation and Tribological Performace of the Complex of β- Cyclodextrin and RC2540 Dialkyl Pentasulfide
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Study on Subcritical Water Extraction of Flavonoids from Scutellaria baicalenses
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Study on Preparation and Characterization of Modified Montmorillonite with the Aromatic Isocyanate
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Determination L-Arginine from Grape Juice with Sakaguchi Reaction
p.301

Effect of Folin Reagent from Different Sources on Protease Determination
p.305

Influence of Chromium on CO₂/H₂S Corrosion Behavior of X100 Grade Pipeline Steel
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Texture Characterization of Low- and Full-Fat Cheese Fermented by Lactobacillus acidophilus during Ripening Process
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Experimental Study on High-Speed WEDM Finishing in Gas Based on Central Composite Design
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Influence of Sintering Temperature on Performance of Red Mud Lightweight Ceramsite
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HomeAdvanced Materials ResearchAdvanced Materials Research Vol. 662Effect of Folin Reagent from Different Sources on...

Effect of Folin Reagent from Different Sources on Protease Determination

Abstract:

Folin reagent was one of the most important reagents in protease determination. The differences between Folin reagent from laboratory and Folin reagent sold were studied. The results showed that there was not significant difference between the absorbance of Folin reagent from lab replaced 30d and Folin reagent sold (p<0.05). The RSD of Folin reagent from lab was between 0.3and 1.67, and Folin reagent sold between 0.63 and 1.49. The precision of them was both higher. The maximum absorption wave length of the chromogenic agent of Folin reagent was with 736-755. And there was no significant difference of the value of the same sample protease between both Folin reagent (p<0.05). Folin reagent from lab based on GB/T23527-2009 was perfect.