A Highly Sensitive Resonance Rayleigh Scattering Method for Platelet-Derived Growth Factor Using Aptamer-Nanogold Probe as Catalyst of the Cu₂O Particle Reaction

Abstract:

Using sodium citrate as reducing agent, different sizes of gold nanoparticles(GN) were prepared, and were modified by platelet-derived growth factor (PDGF) aptamer to obtain stable aptamer-nanogold probes (Apt-GN). The probe specifically combined with PDGF-AA to form Apt-GN-PDGF-AA clusters that exhibited a resonance Rayleigh scattering (RRS) peak at 550 nm. The RRS intensity ΔI_550nm was linear to the PDGF-AA concentration in the range of 0.33-40 ng/ml. The probe has strong catalysis of the Fehling reagent-glucose Cu₂O particle reaction that can be monitored by RRS technique at 610 nm, but the cluster is very weak. When PDGF-AA concentration increased, the Apt-GN decreased, and the RRS intensity at 610 nm decreased. The decreased RRS intensity ΔI_610nm was linear to PDGF-AA concentration in the range of 0.03-26.67 ng/mL. Accordingly, two new aptamer-nanogold RRS methods were established.

• Periodical: Advanced Materials Research (Volume 680)
• Pages: 137-140
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.680.137
• Online since: April 2013
• Authors: Dong Mei Yao, Gui Qing Wen, Jian Niao Tian, Zhi Liang Jiang, Ai Hui Liang
• Keywords: Aptamer, Fehling Reagent, Nanogold Catalysis, PDGF-AA, Resonance Rayleigh Scattering

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