An Immunonanosilver Resonance Rayleigh Scattering Spectral Probe for Rapid Assay of Human Chorionic Gonadotrophin

Abstract:

In pH 6.6 Na₂HPO₄- citric acid buffer solution and in the presence of KCl, the immunoreaction between hCG and nanosilver-labeled anti-hCG took place, the immunonanosilver-complex was formed and deposited, caused the resonance Rayleigh scattering (RRS) intensity at 510 nm decreased. In the optimal condition, the decreased RRS intensity responds linearly with the concentration of hCG over 0.125-1.75 µg/mL. Based on this, a new and simple RRS method has been proposed for the determination of hCG in serum samples, with satisfactory results.

• Periodical: Advanced Materials Research (Volume 680)
• Pages: 141-144
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.680.141
• Online since: April 2013
• Authors: Qing Ye Liu, Gui Qing Wen, Kun Li, Ai Hui Liang
• Keywords: Human Chorionic Gonadotrophin, Immunoassay, Nanosilver-Labeled Anti-hCG, Resonance Rayleigh Scattering

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