Production of Herbal Nanopowders: Effect of Milling Time

Abstract:

Several clinical studies have proved the triterpenes in this herb have therapeutic properties and posses significant to health such as antitumor and wound healing. Centella asiatica nanopowders (CANPs) have been produced via top down approach using Planetary Ball Mill (PBM) at 0.5, 4 and 8 hours of milling in optimized condition. CANPs have been characterized using Photo Correlation Spectroscopy (PCS), Field Emission Scanning Electron Microscope (FESEM) and High Performance Liquid Chromatography (HPLC). As a result significant reduction on size was observed as increased a milling time by comparing their z-Average (nm) and the morphology of CANPs exist in the agglomerated form. The HPLC results showed significant increase in concentration of asiatic acid by comparing the concentration of asiatic acid in extract of CANPs and coarse powders whereby the asiaticoside completely disappeared after milling. Thus, nanoherbal can be said as improving the extraction ratio of asiatic acid compared to the coarse powders due to the smaller particle size and larger surface area and the disappearance of asiaticoside still not completely understood.

• Periodical: Advanced Materials Research (Volume 686)
• Pages: 3-7
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.686.3
• Online since: April 2013
• Authors: M.Z. Borhan, A. Norhidayah, R. Ahmad, Mohamad Rusop, Saifollah Abdullah
• Keywords: Asiatic Acid, Ball Mill, Centella asiatica, Nanopowder

References

[1] C. J. Zheng and L. P. Qin, Chemical components of Centella asiatica and their bioactivities, Journal of Chinese Integrative Medicine, 5 (2007) 348-351.

DOI: 10.3736/jcim20070324

[2] B. C. Park, K. O. Bosire, E. S. Lee, Y. S. Lee, and J. A. Kim, Asiatic acid induces apoptosis in SK-MEL-2 human melanoma cells, Cancer Letters, 218 (2005) 81-90.

DOI: 10.1016/j.canlet.2004.06.039

[3] M. N. Nasir, J. Abdullah, M. Habsah, R. I. Ghani, and G. Rammes, Inhibitory effect of asiatic acid on acetylcholinesterase, excitatory post synapticpotential and locomotor activity, Phytomedicine, 19 (2012) 311-316.

DOI: 10.1016/j.phymed.2011.10.004

[4] R. Rajkhowa, L. Wang, J. Kanwar, and X. Wang, Fabrication of ultrafine powder from eri silk through attritor and jet milling, Powder Technology, 191 (2009) 155-163.

DOI: 10.1016/j.powtec.2008.10.004

[5] P. Y. Ma, Z. Y. Fu, Y. L. Su, J. Y. Zhang, W. M. Wang, H. Wang, Y. C. Wang, and Q. J. Zhang, Modification of physicochemical and medicinal characterization of Liuwei Dihuang particles by ultrafine grinding, Powder Technology, 191 (2009) 194-199.

DOI: 10.1016/j.powtec.2008.10.008

[6] P. K. Inamdar, R. D. Yeole, A. B. Ghogare, and N. J. De Souza, Determination of biologically active constituents in Centella asiatica, Journal of Chromatography A, 742 (1996) 127-130.

DOI: 10.1016/0021-9673(96)00237-3

[7] J. Lee, Drug nano- and microparticles processed into solid dosage forms: Physical properties, Journal of Pharmaceutical Sciences, 92 (2003) 2057-(2068).

DOI: 10.1002/jps.10471

[8] A. A. Noyes and W. R. Whitney, The rate of solution of solid substances in their own solutions, The Journal of the American Chemical Society, 19 (1897) 930-934.

DOI: 10.1021/ja02086a003

[9] Y. L. Su, Z. Y. Fu, W. M. Wang, H. Wang, Y. C. Wang, J. Y. Zhang, and P. Y. Ma, Ultrafine grinding of Radix salvia miltiorrhiza particles and the physicochemical properties by high speed centrifugal sheering, vol. 330-332 I, ed, 2007, pp.215-218.

DOI: 10.4028/www.scientific.net/kem.330-332.215