Effects of Enzyme Concentration, pH, Time and Temperature on the β-Galactosidase Activity Immobilized by Chitosan Beads with Material Properties

He Chen; Juan Wang; Yi Chao Dang; Guo Wei Shu

doi:10.4028/www.scientific.net/AMR.700.251

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HomeAdvanced Materials ResearchAdvanced Materials Research Vol. 700Effects of Enzyme Concentration, pH, Time and...

Effects of Enzyme Concentration, pH, Time and Temperature on the β-Galactosidase Activity Immobilized by Chitosan Beads with Material Properties

Abstract:

In the present study, the experiments were investigated with the effect of enzyme concentration, pH, time and temperature on the enzyme activity of the immobilized β-galactosidase on Chitosan beads with material properties. The enzyme activity will be measured after completing immobilized and expressed as the absorbency at the wavelength of 420 nm. In the process, there were major differences between the different conditions during immobilization. As the result,the optimal conditions were that adding chitosan beads into 8 mg/ml β-galactosidase solution (pH6.5) and adsorbed 6 h at 25°C.

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Advanced Materials Research (Volume 700)

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251-254

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https://doi.org/10.4028/www.scientific.net/AMR.700.251

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Online since:

May 2013

Authors:

He Chen, Juan Wang, Yi Chao Dang, Guo Wei Shu

Keywords:

Chitosan, Enzyme Activity, Immobilized Enzyme, β-Galactosidase

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References

[1] J.Kocin: Lactose intolerance. Intertational Journal of Biochemistry, Vol.20 (1), 1988,pp.1-5

Google Scholar

[2] Quinn Z.K. Zhou, Xiao Dong Chen: Effects of temperature and pH on the catalytic activity of the immobilized β-galactosidase from Kluyveromyces lactis. Biochemical Engineering Journal, Vol. 9 (2001), p.33–40

DOI: 10.1016/s1369-703x(01)00118-8

Google Scholar

[3] V.P Torchilin: Immobilised enzymes as drugs. Advanced Drug Delivery Reviews, Vol.1 (1987), pp.41-86.

DOI: 10.1016/0169-409x(87)90068-8

Google Scholar

[4] Valentina Nichele, Michela Signoretto, Elena Ghedini: β-Galactosidase entrapment in silica gel matrices for a more effective treatment of lactose intolerance. Journal of Molecular Catalysis B: Enzymatic, Vol. 71 (2011), pp.10-15

DOI: 10.1016/j.molcatb.2011.03.002

Google Scholar

[5] Jayakumara, R., Prabaharanb, M., Naira, S. V., Tamura, H.: Novel chitin and chitosan nanofibers in biomedical applications. Biotechnology Advances, 28(1), (2010), p.142–150

DOI: 10.1016/j.biotechadv.2009.11.001

Google Scholar

[6] Janes, K. A., Calvo, P.,Alonso, M. J. Polysaccharide colloidal particles as deliverysystems for macromolecules. Advanced Drug Delivery Reviews, Vol.47 (2001), p.83–97.

DOI: 10.1016/s0169-409x(00)00123-x

Google Scholar

[7] W.S. Wan Ngah, S. Fatinathan. Adsorption of Cu (II) ions in aqueous solution using chitosan beads,chitosan–GLA beads and chitosan–alginate beads. Chemical Engineering Journal, Vol. 143 (2008), p.62–72

DOI: 10.1016/j.cej.2007.12.006

Google Scholar

[8] D. Cavaille, D. Combes: Characterization of beta-galactosidase from Kluyveromyces lactis, Biotechnol. Appl. Biochem. Vol.22 (1) (1995), p.55–64.

Google Scholar