Antioxidant Activities of Tilapia (Oreochromis niloticus) Protein Hydrolysates-Iron Chelate Based on Properties of Biological Materials

Xiao Hu; Sai Rui Ma; Yan Yan Wu; Lai Hao Li; Xian Qing Yang; Shu Xian Hao; Sheng Jun Chen; Wan Ling Lin

doi:10.4028/www.scientific.net/AMR.700.263

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Antioxidant Activities of Tilapia (Oreochromis niloticus) Protein Hydrolysates-Iron Chelate Based on Properties of Biological Materials

Abstract:

Tilapia (Oreochromis niloticus) protein was hydrolysed by papain, and then the tilapia (Oreochromis niloticus) protein hydrolysates (TPH) chelated with ferrous ions at various mass ratios of TPH to FeCl₂(5:1, 10:1 and 20:1) to obtain the biological materials of chelate I, chelate II and chelate III, respectively. The ferrous-binding rate and antioxidant activities of the chelates were investigated. It was found that the ferrous-binding rate increased with the increase of mass ratios (TPH/FeCl₂) from 5:1 to 20:1. The DPPH radical scavenging activity and reducing power activity of chelate III were highest in the chelates, and the lipid peroxidation inhibition activity of the chelate III was obviously higher than that of TPH. Compared with TPH, marked changes in microstructure were observed in the biological material of TPH-iron chelate.