Effect of Four Materials Including Trehalose, Soluble Starch, Raffinose and Galactose on Survival of Lactobacillus acidophilus during Freeze-Drying

Abstract:

Effect of four materials including trehalose, soluble starch, raffinose and galactose on survival of Lactobacillus acidophilus was studied by counting viable cells before and after freeze-drying. The concentrations of five carbohydrates were all 3%, 6%, 9%, 12% and 15%. Results showed as follows: the optimal concentrations of trehalose, soluble starch, raffinose and galactose were 12%, 9%, 3% and 15%, the survival of Lactobacillus acidophilus was 42.62%, 56.50%, 45.05% and 42.69% and the viable cells of lyophilized powder were 0.40×10¹¹cfu/g,0.45×10¹¹cfu/g, 0.52×10¹¹ and 1.09×10¹¹cfu/g, respectively.

• Periodical: Advanced Materials Research (Volume 700)
• Pages: 259-262
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.700.259
• Online since: May 2013
• Authors: Tao Qin, Qi Ma, He Chen, Guo Wei Shu
• Keywords: Carbohydrate, Cryoprotectant, Freeze-Drying, Lactobacillus acidophilus, Viable Cells

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