For Libraries For Publication Open Access Downloads About Us Contact Us
Paper Titles
Preface
Studies of Chemical Adsorption Membrane in the Steel Surface on Application
p.3
Synthesis of (S)-(+)-1,3-butanediol by asymmetric reduction of4-hydroxy-2-butanone with Immobilized yeast cells
p.7
Synthesis of (S)-Tert-Butyl 3-Hydroxybutyrate by Asymmetric Reduction of Tert-Butyl Acetoacetate with Saccharomyces cerevisiae B5
p.12
Supercritical Fluid Extraction of Polygonum cuspidatum and Subsequent Isolation by Simulated Moving Bed Chromatography
p.18
Anodic Plasma Electrolytic Nitrocarburizing of Low-Carbon Steel
p.31
Anodic Plasma Electrolytic Saturation of Steels by Carbon and Nitrogen
p.37
Interaction Mechanism between Bovine Serum Albumin and Polyethylene Glycol Revealed with Elastic Light Scattering Spectroscopy
p.43
COD Removal of Textile Printing and Dyeing Wastewater by Photocatalysis
p.51

Supercritical Fluid Extraction of Polygonum cuspidatum and Subsequent Isolation by Simulated Moving Bed Chromatography

Article Preview

Abstract:

In this work, supercritical carbon dioxide with ethanol as cosolvent was employed to extract bioactive compounds from Polygonum Cuspidatum. Effect of temperature for 40 and 50 C on the extraction at 35 MPa was examined. It was found that extraction at 50 C resulted in high yield. A two-stage extraction at 50 C and 35 MPa was then designed to investigate effect of cosolvent on the recovery of resveratrol and emodin. The first stage extraction by solely using carbon dioxide can rarely extract resveratrol, but adding ethanol as cosolvent in the second stage significantly increases the extraction of resverarol and emodin. The crude extract obtained in the second stage was very stable against agglomeration and precipitation, because the first stage extraction helped to remove impurities from the Polygonum Cuspidatum. The stable crude extract was subsequently used as the feed for the SMB (simulated moving bed) separation. In this work, experimental validation was completed by solely using single SMB to isolate resveratrol, emodin, and physcion from the crude extract. As conducting the SMB experiments, the Triangle theory was used to help to determine the separable operating conditions. From the discussion of the experimental results, the criterion to obtain the pure resveratrol and physcion, and the concentrated emodin were depicted. Since both the extraction and the purification are conducted at low temperature, the bioactivity of the produced natural products will totally be preserved. The two-stage extraction and subsequent purification in this paper will provide greener and effective alternative for the development of botanical drugs.

You might also be interested in these eBooks
2013 Spring International Conference on Material Sciences and Technology (MST-S) View Preview

Info:

Periodical:

Advanced Materials Research (Volume 704)

Pages:

18-30

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.704.18

Citation:

Cite this paper

Online since:

June 2013

Authors:

Ming Tsai Liang, Ru Chien Liang, Shu Qi Yu, Ri An Yan, Ku Yuan Liang

Keywords:

Polygonum cuspidatum, Resveratrol, Simulated Moving Bed Chromatography, Supercritical Fluid Extraction

Export:

RIS, BibTeX

Price:

Permissions CCC:

Request Permissions

Permissions PLS:

Request Permissions

Сopyright:

© 2013 Trans Tech Publications Ltd. All Rights Reserved

Share:

Citation:

References

[1] B.B. Aggarwal , A. Bhardwaj, R.S. Aggarwal, N.P. Seeram, S. Shishodia, Y. Takada, Role of resveratrol in prevention and therapy of cancer: preclinical and clinical studies, Anticancer Res. 24(5a) (2004) 2783-2840.

Google Scholar

[2] S. Shankar, G. Singh, R.K. Srivastava, Chemoprevention by resveratrol: molecular mechanisms and therapeutic potential, Front Biosci. 12 (2007) 4839-4854.

DOI: 10.2741/2432

Google Scholar

[3] J.S. Chang, H.W. Liu, K.C. Wang, M.C. Chen, L.C. Chiang, Y.C. Hua, C.C. Lin, Ethanol extract of Polygonum cuspidatum inhibits hepatitis B virus in a stable HBV-producing cell line, Antiviral Res. 66(1) (2005) 29-34.

DOI: 10.1016/j.antiviral.2004.12.006

Google Scholar

[4] H.Z. Zheng, Z.H. Dong, J. She, H. Zhang, Rhizoma polygoni cuspidate, in: modern study of traditional Chinese Medicine, vol. 3, Xue Yuan Press, Beijing, 1998, p.2800

Google Scholar

[5] X. Chu, A. Sun, R. Liu, Preparative isolation and purification of five compounds from the Chinese medicinal herb Polygonum cuspidatum Sieb. et Zucc by high-speed counter-current chromatography, J. Chromatogr. A 1097 (2005) 33-39.

DOI: 10.1016/j.chroma.2005.08.008

Google Scholar

[6] L. Chen, Y.H. Han, F.Q. Yang, T.Y. Zhang, High-speed counter-current chromatography separation and purification of resveratrol and piceid from polygonum cuspidatum, J. Chromatogr. A 907 (2001) 343-346.

DOI: 10.1016/s0021-9673(00)00960-2

Google Scholar

[7] F.Q. Yang, T.Y. Zhang, Y.C. Ito, Large-Scale separation of resveratrol, anthraglycoside A and anthraglycoside B from polygonum cuspidatum Sieb. Et Zucc by high-speed counter-current chromatography, J Chromatogr. A 919 (2001) 443-448.

DOI: 10.1016/s0021-9673(01)00846-9

Google Scholar

[8] J.C. Zhang, L.J. Gao, Purificaiton of resveratrol by simulated moving bed chromatography, Chinese Journal of Spectroscopy Laboratory 29 (2012) 150-152.

Google Scholar

[9] J.C. Zhang, L.J. Gao, Pretreatment of raw materials for purification of resveratrol by simulated moving bed chromatography, Chinese Journal of Spectroscopy Laboratory 28 (2011) 1763-1766.

Google Scholar

[10] H.M. Lu , W.D. Ni, Y.Z. Liang, R.L. Man, Supercritical CO2 extraction of emodin and physcion from Polygonum cuspidatum and subsequent isolation by semipreparative chromatography, J. Sep. Sci. 29 (2006) 2136-2142.

DOI: 10.1002/jssc.200600012

Google Scholar

[11] W.D. Ni, R.L. Man, L.Y. Li, X.Y. Ji, R.X. Shi, S.Q. Yang, S. Zhang, Study on the supercritical carbon dioxide extraction effective constituents from Chinese herb Polygonum cuspidatum, Chemical Engineer 132 (2006) 3-7 (in Chinese).

Google Scholar

[12] W.L. Yu, , B. Shu, Y.P. Zhao, Supercritical CO2 extraction of resveratrol and its glycoside piceid from Chimese Traditional Medicinal Herb Polygonum cuspidatum, J. Sci. Food Agric., 85 (2005) 489-492.

DOI: 10.1002/jsfa.2007

Google Scholar

[13] B. Benova, M. Adam, P. Pavlikova, J. Fischer, Supercritical Fluid Extraction of Piceid, resveratrol and emodin from Japanese knotweed, J. Supercrit. Fluids 51 (2010) 325-330.

DOI: 10.1016/j.supflu.2009.10.009

Google Scholar

[14] A. Rajendran, G. Paredes, M. Mazzotti, Simulated Moving Bed Chromatography for the Separation of Enantiomers, J Chromatogr. A 1216 (2009) 709-738.

DOI: 10.1016/j.chroma.2008.10.075

Google Scholar

[15] H. Schmidt-Traub (Eds.), Preparative Chromatography of Fine Chemicals and Pharmaceutical Agents, Wiley-VCH Verlag GmbH & Co., KGaA, Weinheim, 2005.

DOI: 10.1002/3527603484

Google Scholar

[16] G. Subramanian, Chiral Separation Techniques – A Practical Approach, 2nd ed., Wiley-VCH, Verlag GmbH & Co. , KGaA, Weinheim, 2001.

DOI: 10.1021/ja076917g

Google Scholar

[17] M.T. Liang, R.C. Liang, L.Y. Wang, H.E. Yen, K.T. Lee, Effect of Temperature Variation on the Separation of Sesamin and Sesamolin by Simulated Moving Bed, J. Chem. Chem. Eng. 5(6) (2011) 479-786.

Google Scholar

[18] J.X. Cong, B.C. Lin, Separation of Liquiritin by simulated moving bed chromatography, J Chromatogr. A 1145 (2007) 190-194.

DOI: 10.1016/j.chroma.2007.01.088

Google Scholar

[19] F. Wei, Y.X. Zhao, Separation of capsaicin from capsaicinoids by simulated moving bed chromatography, J Chromatogr. A 1187 (2008) 281-284.

DOI: 10.1016/j.chroma.2007.12.081

Google Scholar

[20] M.T. Liang, R.C. Liang, L.R. Huang, P.H. Hsu, Y.H. Wu, H.E. Yen, Separation of Sesamin and Sesamolin by a Supercritical Fluid-Simulated Moving Bed, Am. J. Anal. Chem. 3 (2012) 931-938.

DOI: 10.4236/ajac.2012.312a123

Google Scholar

[21] P.J. Dunn, A.S. Wells, M.T. Williams (Eds.), Green Chemistry in the pharmaceutical industry, Wiley-VCH, Verlag GmbH & Co. , KGaA, Weinheim, 2010.

Google Scholar

[22] M.T. Liang, R.C. Liang, H.F. Hsu, J.Y. Houng, Separation of Bioactive Ingredients from Glossogyne tenuifolia Extract by Simulated Moving Bed, The International Conference on Process Intensification for Sustainable Chemical Industries (ICPI 2011), June 26-29, 2011, Beijing, China

Google Scholar

[23] M.T. Liang, R.C. Liang, K.Y. Liang, Separation of Triterpenoids from Taiwanofugus camphorata by Supercritical Fluid Extraction and Simulated Moving Bed, 10th Conference on Supercritical Fluids and Their Applications, April 29-May 06, 2013, Naples, Italy

DOI: 10.1016/j.jtice.2013.10.013

Google Scholar

[24] M.T. Liang, R.C. Liang, L.R. Huang, K.Y. Liang, Y.L. Chien, J.Y. Liao, Supercritical Fluids as the Desorbent for Simulated Moving Bed – Application to the Concentration of Triterpenoids from Taiwanofugus camphorata, 14th International Symposium on Preparative and Industrial Chromatography and Allied Techniques (SPICA 2012), September 30-October 3, 2012, Brussels, Belgium

DOI: 10.1016/j.jtice.2013.10.013

Google Scholar

© 2025 Trans Tech Publications Ltd. All rights are reserved, including those for text and data mining, AI training, and similar technologies. For open access content, terms of the Creative Commons licensing CC-BY are applied.
Scientific.Net is a registered trademark of Trans Tech Publications Ltd.