Study on the Interaction between a Self-Assembling Peptide and Paclitaxe

Abstract:

In this work, the interaction between paclitaxel (PTX) and a designed peptide was investigated through AFM, CD and fluorescence spectroscopy. AFM images showed that the morphology of the self-assembling peptide with PTX addition had a remarkable change. Fluorescence data illustrated that PTX enhanced the fluorescence intensity of P1. The binding constants for the interaction between PTX and P1 in 25°Cand 35°Cwere 6.38×105 L/mol and 2.27×104 L/mol respectively. Moreover, thermodynamic analysis implied the main binding force between P1 and PTX was hydrogen bonds and vander Waals forces. CD results indicated that PTX caused an increase of unordered structure. Finally, a molecular modeling was proposed based on the research. The study will provide a reliable proof for designed peptide as a paclitaxel carrier for further research.

• Periodical: Advanced Materials Research (Volumes 706-708)
• Pages: 284-291
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.706-708.284
• Online since: June 2013
• Authors: Chen Hui, Zhi Hua Xing, Hong Chang Yu, Li Ping Ruan
• Keywords: Fluorescence, Interaction, Paclitaxel, Peptide, Self-Assemble

References

[1] Stuart Kyle, Amalia Aggeli, Eillen lngham and Michaael J.McPherson. Production of self-assembling biomaterials for tissue engineering [J]. Trends in Biotechnology, 2009, 27(7):423-434.

DOI: 10.1016/j.tibtech.2009.04.002

[2] Paola Brun, Francesca Ghezzo, Martina Roso, Roberta Danesin, Giorgio Palù, Andrea Bagno, Michele Modesti, Ignazio Castagliuolo and Monica Dettin. Electrospun scaffolds of self-assembling peptides with poly (ethylene oxide) for bone tissue engineering [J]. Acta Biomaterialia, 2011, 7:2526-2533.

DOI: 10.1016/j.actbio.2011.02.025

[3] Archana Mishra, Yihua Loo, Rensheng Deng, Yon Jin Chuah,Hwan Tak Hee, Jackie Y. Ying and CharlotteE.Hauser. Ultrasmall natural peptides self-assemble to strongtemperature-resistant helical fibers in scaffolds suitable for tissue engineering [J].nanotoday, 2011, 6:232-239.

DOI: 10.1016/j.nantod.2011.05.001

[4] E. Mateo-Mart, C. Briones, C.M. Pradier, J.A. Mart ´ın-Gago. A DNA biosensor based on peptide nucleic acids on gold surfaces [J].Biosensors Bioelectronics, 2007, 22:1926-1932.

DOI: 10.1016/j.bios.2006.08.012

[5] Honglan Qi, Chen Wang, Xiaoying Qiu, Qiang Gao and Chengxiao Zhang. Reagent-less electrogenerated chemiluminescence peptide-based biosensor for the determination of prostate-specific antigen [J].talanta, 2012, 100(15):162-167.

DOI: 10.1016/j.talanta.2012.08.037

[6] Zhang, H., Luo, H. and Zhao Xiaojun. Mechanistic Study of Self-Assembling Peptide RADA16-I in Formation of Nanofibers and Hydrogels [J]. Journal of Nanotechnologyin Engineering and Mediciner, 2010, 1(1):7-13.

DOI: 10.1115/1.4000301

[7] Liping Ruana, Hangyu Zhanga, Hanlin Luoa, Jingping Liua, Fushan Tanga, Ying-Kang Shib and Xiaojun Zhao. Designed amphiphilic peptide forms stable nanoweb,slowly releases encapsulated hydrophobic drug, andaccelerates animal hemostasis [J]. PNAS, 2009, 106(13): 5105-5110.

DOI: 10.1073/pnas.0900026106

[8] Li-Ping Ruan, Han-Lin Luo, Hang-Yu Zhang and Xiaojun Zhao. Investigation on Structure and Properties of a Novel Designed Peptide with Half-Sequence Ionic Complement [J]. Macromolecular Research, 2009, 17(8):597-602.

DOI: 10.1007/bf03218915

[9] Li-Ping Ruan, Hang-Yu Zhang, Han-Lin Luo and Xiaojun Zhao. Application research of a novel designed peptide as a potential carrier [J]. Science in China Series B: Chemistry, 2009, 17(8):597-602

[10] Pandita D, Ahuja A and Lather V, et al. Development of lipidbased nanoparticles for enhancing the oral bioavailability of paclitaxel [J]. AAPS PharmSciTech, 2011, 12: 712-722.

DOI: 10.1208/s12249-011-9636-8

[11] Pan LL, Dong J, Wang SL. Preparation and preliminary evaluation of physical stability of the paclitaxel injection [J]. J Shenyang Pharm Univ, 2004, 21: 101-104.

[12] Sun Bingfeng, Ranganathan Balu, Feng Si-Shen. Multi-functional poly(D, L-lactide-co-glycolide) /montmorillonite (PLGA/MMT) nanoparticles decorated by Trastuzumab for targeted chemotherapy of breast cancer [J].Biomaterials, 2008, 29:475-486

DOI: 10.1016/j.biomaterials.2007.09.038

[13] Liu Jiang, Meisner Dale, Kwongb Elizabeth, et al. A novel trans-lymphatic drug delivery system: Implantable gelatin sponge impregnated with PLGA-paclitaxel microspheres[J].Biomaterials, 2007, 28:3236-3244

DOI: 10.1016/j.biomaterials.2007.03.022

[14] Sung Chul Kim, Dong Wook Kim, Yong Ho Shim, et al. In vivo evaluation of polymericmicellar paclitaxel formulation: toxicity and efficacy[J].Journal of Controlled Release, 2001, 72:191-202.

DOI: 10.1016/s0168-3659(01)00275-9

[15] Rajagopal Subramanyama, Anilkishor Gollapudia, Persis Bonigalaa, Madhurarekha Chinnaboinaa and Damu G. Amoorub. Betulinic acid binding to human serum albumin: A study of protein conformation and binding affinity [J]. Journal of Photochemistry and Photobiology B: Biology, 2009, 94(1):8-12.

DOI: 10.1016/j.jphotobiol.2008.09.002

[16] Ying Yi, Wen Ying He, YuMing Dong, Fenling Sheng and ZhiDe Hu.Human serum albumin interaction with formononetin studied using fluorescence anisotropy, FT-IR spectroscopy, and molecular modeling methods [J]. Bioorganic & Medicinal Chemistry, 2006, 14(5):1431-1436.

DOI: 10.1016/j.bmc.2005.09.066

[17] Fengling Cui, Yinghua Yan, Qiangzhai Zhang, Juan Du, Xiaojun Yao, Guirong Qu and Yan Lu. Characterization of the interaction between 2'-deoxyuridine and human serum albumin [J]. Carbohydrate Research, 2009, 344(5):642-647.

DOI: 10.1016/j.carres.2009.01.009

[18] Yan-Jun Hu, Yi Liu, Xue-song Shen, Xian-Yang Fang and Song-Sheng Qu. Studies on the interaction between 1-hexylcarbamoy1-5-fluorouracil and bovine serum albumin [J]. Spectrochimica Acta Part A: Molecular and Bimolecular Spectroscopy, 2012, 85:79-84.

[19] Zhang Xi，Kou Zi-nong,Shi Yu-jia,Zhu Jing-bo, Investigation on effect of Fluorecence Enhancement of Danshensu-BSA ststem by synchronous fluorescence spectrometry.[J]. Journal of instrumental Analysis, 2011, 4:444-447.