Fabrication and Characterization of Vitamin E TPGS Loaded Silk Fibroin/Hyaluronic Acid Nanofibrous Scaffolds

Abstract:

Vitamin E d-alpha-tocopheryl polyethylene glycol 1000 succinate (VE TPGS) loaded silk fibroin (SF)/ hyaluronic acid (HA) nanofibrous scaffolds were fabricated by means of electrospinning to biomimic the natural extracellular matrix. Scanning electronic microscopy (SEM) results indicated that electrospun VE TPGS loaded SF/HA nanofibers were ribbon-shaped, the width of nanofibers decreased slightly with the addition of VE TPGS to SF/HA blended solutions. Fourier transform infrared (FTIR) spectroscopy and Wide-angle X-ray diffraction (WAXD) curves revealed that VE TPGS did not induce SF conformation from random coil to β-sheet. SF conformation converted from random coil to β-sheet after being treated with 75% ethanol vapor. In vitro release studies confirmed VE TPGS had no obvious burst release and present good release behavior.

• Periodical: Advanced Materials Research (Volume 721)
• Pages: 274-277
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.721.274
• Online since: July 2013
• Authors: Li Li Ji, Qiao Ling Li, Zeng Hu Yang, Wei Jing Hu, Kui Hua Zhang
• Keywords: Electro-Spinning, Nanofiber, Silk Fibroin/Hyaluronic Acid, Vitamin E TPGS

References

[1] B. M. Min, G. Lee, S. H. Kim, Y. S. Nam, and W. H. Park: Biomaterials, Vol. 25(2004), p.1289

[2] M. Wang, H.J. Jin, L. David and G. C. Rutledge: Macromolecules, Vol. 37 (2004), p.6856

[3] W. J. Li, C. T. Laurencin, E. J. Caterson, R. S. Tuan and F. K. Ko: J. Biomed. Mater. Res. , Vol. 60 (2002), p.613

[4] M. Santin, A. Motta, G. Freddi and M. Cannas: J. Biomed. Mater. Res. Vol. 46(1999), p.382

[5] R. L. Horan, K. Antle, A. L. Collette, and G. H. Altman: Biomaterials, Vol.26(2005), p.3385

[6] S. M. Kuo, Y. J. Wang, G. C. Niu, H. E. Lu and S. J. Chang, J. Mater: Sci. Mater. Med., Vol.19(2008), p.1235

[7] R. E. Beyer, J. Bioenerg. Biomembrance, Vol. 26(1994), p.349