Development of a Highly Sensitive Chemiluminescence Enzyme Immunoassay for the Determination of Bisphenol A

Abstract:

A highly sensitive chemiluminescence enzyme immunoassay (CLEIA) method for the determination of bisphenol A (BPA) was developed, which used a secondary antibody labeled with horseradish peroxidase detected with a luminol-based substrate. Under the optimized conditions, the linear range was 0.01μg/mL~0.74μg/mL, and the detection limit was 0.008μg/mL. The average recovery for BPA in barreled water was 104%. This developed method could be applied for the selective, high-throughput, and rapid determination of BPA in barreled water.

• Periodical: Advanced Materials Research (Volumes 726-731)
• Pages: 1283-1286
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.726-731.1283
• Online since: August 2013
• Authors: Li Rui Liu, Li Qin Liu, Xue Qing Chen, Guo Qing Shi
• Keywords: Biphenol A, Chemiluminescence Enzyme Immunoassay, Determination

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