Determination of Chlorophyll in Water by Fluorophotometry with Chlorophyll-A Extraction from Microcystis aeruginosa Instead of Chlorophyll Standard Substance

Jian Sheng Cui; Yu Xin Cheng; Xiao Hui Xu

doi:10.4028/www.scientific.net/AMR.726-731.1269

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HomeAdvanced Materials ResearchAdvanced Materials Research Vols. 726-731Determination of Chlorophyll in Water by...

Determination of Chlorophyll in Water by Fluorophotometry with Chlorophyll-A Extraction from Microcystis aeruginosa Instead of Chlorophyll Standard Substance

Abstract:

Microcystis aeruginosa is common in freshwater lakes, and developed easy. Instead of chlorophyll standard substance, fresh chlorophyll extraction from Microcystis aeruginosa applied to the determination of chlorophyll-a in water by fluorophotometry. Thus the relationship between concentration of chlorophyll-a and fluorescence intensity of Microcystis aeruginosa in water was established. The fluorescence intensity responded with the concentration of chlorophyll-a extract linearly in the range of 30~1800 ug/L with a correlative coefficient of 0.9947. Comparision was made between chlorophyll standard and the chlorophyll-a extraction of Microcystis aeruginosa, and no significant difference was found. In certain range of pH value and time, chlorophyll-a extraction from Microcystis aeruginosa is stable, and can be used to reflect the growth of algae in water quick, conveniently and accurately.

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Periodical:

Advanced Materials Research (Volumes 726-731)

Pages:

1269-1274

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.726-731.1269

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Online since:

August 2013

Authors:

Jian Sheng Cui, Yu Xin Cheng, Xiao Hui Xu

Keywords:

Chlorophyll Extraction, Chlorophyll Standard Substance, Fluorophotometry, Microcystis aeruginosa

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