Measurement of 15N and 18O Isotope Abundance of Nitrate Using Denitrifier Method on Tracegas-Isotope Ratio Mass Spectrometry

Chun Ying Xu; Yu Zhong Li; Qiao Zhen Li; Yi Wei Dong; Fu Li Fang; Zhi Cheng Guo

doi:10.4028/www.scientific.net/AMR.726-731.1346

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HomeAdvanced Materials ResearchAdvanced Materials Research Vols. 726-731Measurement of 15N and 18O Isotope Abundance of...

Measurement of ¹⁵N and ¹⁸O Isotope Abundance of Nitrate Using Denitrifier Method on Tracegas-Isotope Ratio Mass Spectrometry

Abstract:

A modified denitrifier method on Tracegas-isotope ratio mass spectrometry (IRMS) was developed to determine the nitrogen and oxygen isotopic compositions of nitrate (NO₃^-) in water samples. The protocol presented in our previous publication was modified in several aspects. Then, the incubation time, amount of NO₃^- sample, and effect of trapping time in the Tracegas system were analyzed using the standard reference USGS34. Both δ¹⁵N and δ¹⁸O values demonstrated accurate and reproducible at incubation times exceeding 12 h or overnight as well as added 0.2 μg to 0.4 μg NO₃^--N samples. Our data also showed that 100 s for trapping time in Tracegas system was adequate to achieve accurate and reproducible results. Finally, the δ¹⁵N and δ¹⁸O values of nitrate in animal wastewater were measured using this method. The results indicate that the denitrifier method can be used widely for tracing nitrate sources and controlling nitrate contamination in China.

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Periodical:

Advanced Materials Research (Volumes 726-731)

Pages:

1346-1349

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.726-731.1346

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Online since:

August 2013

Authors:

Chun Ying Xu, Yu Zhong Li, Qiao Zhen Li, Yi Wei Dong, Fu Li Fang, Zhi Cheng Guo

Keywords:

Denitrifier Bacterial, Nitrate, Tracegas Isotope Ratio Mass Spectrometry, δ¹⁵N, δ¹⁸O

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