Degradation of Long-Chain n-Alkanes by Acinetobacter Sp.

Abstract:

Alkanes are widespread in nature and cause seriously pollution of environment. In many of oil pollution treatment method, because of economic, effective and less damaging to the environment and many other advantages, bioremediation technology is thought to have broad application prospects. In this study, we isolated an efficient alkane-degrading strain from some coal gasification wastewater sludge. It was identified the strain L2-4 as Acinetobacter sp.(KC211013) through morphology and 16SrDNA sequence analysis. The maximum degradation rate was aquired to 58.7% under the condition of original alkanes concentration 700mg/L, pH 7.0 and temperature 35°C. The study was designed to provide good strain resources for oil pollution remediation and microbial en hanced oil recovery.

• Periodical: Advanced Materials Research (Volumes 726-731)
• Pages: 2151-2155
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.726-731.2151
• Online since: August 2013
• Authors: Yin Song Liu, Hong Jun Han, Fang Fang
• Keywords: Acinetobacter Strain, Biodegradation, Long-Chain N-Alkanes

References

[1] R. Margesin, D. Labbe, F. Schinner, et al. Characterization of hydrocarbon-degrading microbial populations in contaminated and pristine alpine soils. Appl Environ Microbiol. 2003, 69(2): 3085-3092.

DOI: 10.1128/aem.69.6.3085-3092.2003

[2] R.M. Altas. Petroleum biodegradation and oil spill bioremediation[A]. Symposium on Bioremediation and Bioprocessing. Division of Petroleum Chemistry, Inc. 205th National Meeting of American Chemical Society[C]. Denver, USA: Denver, CO, 1993: 236-237.

[3] He Liangju, Wei Dezhou, Zhang Weiqing. Research of Microbial Treatment of Petroleum Contaminated Soil.Techniques and Equipment for Environmental Pollution Control. 1999, 7(3): 110-115.

[4] A. Wentzel, T.E. Ellingsen, H.K. Kotlar et al, Throne-Holst M. Bacterial metabolism of long-chain n-alkanes. Appl Microbiol Biotechnol. 2007, 76: 1209-21.

DOI: 10.1007/s00253-007-1119-1

[5] S. Harayama, H. Kishira, Y. Kasai, et al. Petroleum Biodegradation in Marine Environments. J. Molec. Microbiol. Biotechnol. 1999, 1(1): 63-70.

[6] B. Lal, S. Khanna. Degradation of crude oil by Acinetobacter calcoaceticus and Alcaligenes odorans. Journal of Applied Bacteriology. 1996, 81: 355-362.

DOI: 10.1111/j.1365-2672.1996.tb03519.x

[7] Y. Sakai, J.H. Maeng, Y. Tani, et al. Use of long-chain (C13-C44) by an isolate, Acinetobacter sp. M-1. Bioscience. Biotechnology and Biochemistry. 1994, 58: 2128-2130.

DOI: 10.1271/bbb.58.2128

[8] Cao Juan, Xu Zhihui, Li Lingzhi, Shen Biao. Biosurfactant Producing Petroleum Degrader Acinetobacter BHSN. Journal of Ecology and Rural Environn-alkanesment. 2009, 25 (1): 73-78.

[9] S.H. Ko, J.M. Lebeault. Effect of a mixed culture on co-oxidation during the degradation of saturated hydrocarbon mixture. Journal of Applied Microbiology. 1999, 87: 72-79.

DOI: 10.1046/j.1365-2672.1999.00797.x

[10] P. Wongsa, M. Tanaka, A. Ueno et al. Isolation and characterization of novel strains of Pseudomonas aeruginosa and Serratia marcescens possessing high efficiency to degrade gasoline. kerosene, diesel oil, and lubricating oil Current Microbiology. 2004, 49: 415-422.

DOI: 10.1007/s00284-004-4347-y

[11] Z. Bihari, Z. Szabó, A.Szvetnik et al. Characterization of a novel long-chain n-alkane-degrading strain Dietzia sp. E1. Z. Naturforsch. C. 2010, 65: 693-700.

DOI: 10.1515/znc-2010-11-1210

[12] Renren Wu, Zhi Dang, Xiaoyun Yi et al. The effects of nutrient amendment on biodegradation and cytochrome P450 activity of an n-alkane degrading strain of Burkholderia sp. GS3C. Journal of Hazardous Materials. 2011, 186: 978-983.

DOI: 10.1016/j.jhazmat.2010.11.095

[13] Jinzhuang Wu, Hongyu Liu, Guangming Zeng et al. Isolation of Oil Degradation Bacteria and Its Characteristics in Bio-degradation to Diesel Oil. Journal of Agro-Environment Science. 2008, 27(5): 1742-1746.

[14] Ye Liu, Weimin Zhou, Bozhong Mu et al. Degradation Characteristics of Long-chain Aliphatic Alkane degrading Bacteria. Journal of Microbiology. 2005, 25(6): 14-18.