Isolation and Characterization of a Strain of Pseudomonas stutzeri for Coal Biodesulfurization

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A strain of Pseudomonas stutzeri named LH42 was isolated from the petroleum-contaminated soil collected from Liaohe Oil Field, Liaoning, China. The strain was capable of degrading dibenzothiophene (DBT). The bacterium was motile, rod-shaped, and grew optimally at 30°C and initial pH of 7.0. The morphological, physiological characterizations and analysis based on 16S rRNA sequence indicated that this strain was most closely related to Pseudomonas stutzeri (>99% similarity in gene sequence). Coal biodesulfurization of this strain was processed. And a total sulfur removal of 41.5% and organic sulfur removal of 92.9% was obtained within 25 days. Scanning electron microscope (SEM) analysis revealed that the strain was closely attached on the coal surface and the coal was markedly etched.

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Advanced Materials Research (Volumes 726-731)

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2403-2408

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August 2013

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© 2013 Trans Tech Publications Ltd. All Rights Reserved

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