Gene Cloning and Function Analysis Involved in the Biodegradation of Aromatic Compounds by Bacillus NAPZ

Abstract:

A bacterial strain NAPZ, which utilized polycyclic aromatic hydrocarbons (PAHs) as the sole carbon and energy source for growth, was studied on its apparent characteristics, key gene structure and functions. It was preliminarily identified as a Bacillus sp. according to its physiological characteristics and the phylogenetic property of its 16S rRNA gene sequence. Based on the reported RHD gene sequences, a pair of primers was designed to amplify the RHD gene from the genomic DNA of strain NAPZ by PCR. Then, the RHD gene was cloned and sequenced. Based on this sequence and its related sequences in the GenBank database, a multiple alignment was conducted then a phylogenetic tree was constructed with DNAMAN and MEGA software. The analysis revealed that strain NAPZ oxidized PAHs via ring hydroxylating dioxygenase (RHD) pathway. The study provided experimental and theoretical support for revealing the structure and function of key genes as well as the PAHs-degrading pathways in strain NAPZ.

• Periodical: Advanced Materials Research (Volumes 726-731)
• Pages: 373-377
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.726-731.373
• Online since: August 2013
• Authors: Chao Li, Chun Yang Zhang, Sheng Hui Chen, Cong Cong Yu, Zheng Long Wang, Chao Chao Lu, Feng Wang
• Keywords: Bacillus, Biodegradation, Phylogeny, Polycyclic Aromatic Hydrocarbons (PAHs), Ring Hydroxylating Dioxygenase (RHD)

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