Genetic Variation of Erigeron breviscapus (Vant.) Hand-Mazz in Yunnan under Heavy Metals Stress

Abstract:

Erigeron breviscapus (Vant.) Hand-Mazz is the raw materials to product Health drugs and injectable formulations. Study the genetic diversity of Erigeron breviscapus under harmful substances stress provides insight into their genetic variation characteristics under stress. In this research, genetic diversity of Erigeron breviscapus under heavy metals (Cr, Cd, Hg, Cu, Pb) stress with different concentrations were analyzed by using 8 inter-simple sequence repeat markers (ISSR). The results showed that Erigeron breviscapus had rich genetic variation under five different kinds heavy metals stress. A total of 96 bands were amplified, of which 80 polymorphic bands were discovered and the percentage of polymorphic bands (PPB) was 83.33%. Their indexes of Na, Ne, H, I and PPB were calculated. Richer genetic diversity was discovered under Cu stress, followed by Cd, Cr, Pb and Hg stress in order. This study will be provide a scientific basis to improve the quality Erigeron breviscapus and optimize its growing conditions.

• Periodical: Advanced Materials Research (Volumes 726-731)
• Pages: 378-381
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.726-731.378
• Online since: August 2013
• Authors: Xin Xiang A, Hua Bin Xiong, Ming Hong Chen
• Keywords: Erigeron breviscapus, Genetic Diversity, Heavy Metal, ISSR Markers

References

[1] H. Liu, X.L. Yang, H.B. XU: Chinese Traditional and Herbal Drugs. Vol 6. (2002), p.566

[2] J. Zhang, X.S Li, W.D. Zhang: Journal of Pharmaceutical Practice. Vol 2. (2002), p.104

[3] Y.G. Wu, Z.L. Jiang, Q. Luo: Journal of Nanjing Forestry University. Vol 4. (2002), p.39

[4] X.M. Wu, G.X. Pan, H.Y. Jiang, Y.F. Ju: Ecology Environment. Vol 1. (2003), P. 9

[5] C.W. Jin, S.J. Zheng, Y.F. He: Chemosphere. Vol 8. (2005), P. 1151

[6] Y.Z. Shi, L.F. Ma, W.Y. Han: Scientia Agricultural Sinica. Vol 11. (2003), P. 1272

[7] J.Q. Sha, D.X. Zheng: Journal of Tea Science. Vol 2. (1996), P. 141

[8] L.J. Zhou, N.G. Li, H. Yu, S.G. Zhang: Acta Botanica Yunnan. Vol 1. (2005), P. 59

[9] S.C. Yang, Y.F. Li, Z. Zhao, G.S. Wen, Y. Li, Y.G. Liu, Y.J. Yang, F.H. Xiao: China Journal of Chinese Materia Medica. Vol 13. (2008), P. 1532.

[10] J.L. Li, S. Wang, J. Yu, L. Wang, S.L. Zhou: Chinese Bulletin of Botany. Vol 1. (2013), P. 72

[11] M.Z. Yao, H.T. Huang, J.Z. Yu, L. Chen: Journal of Tea Science. Vol 2. (2005), P. 153

[12] M.Z. Yao, L. Chen, X.C. Wang, L.P. Zhao, Y.J. Yang: Acta Agronomica Sinica. Vol 4. (2007), P. 598

[13] F.C. Yeh, R.C. Jang, T.B.J. Boyle, Z.H. Yeh, J.X. Mao: Molecular Biology and Biotechnology Centre. University of Alberta. (1999)