Cloning and Sequence Analysis of Two cDNA Encoding Invertase Inhibitors from Cassava (Manihot esculenta Crantz)

Meng Ting Geng; Yuan Yao; Xiao Hui Wu; Yi Min; Shao Ping Fu; Xin Wen Hu; Jian Chun Guo

doi:10.4028/www.scientific.net/AMR.726-731.4326

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HomeAdvanced Materials ResearchAdvanced Materials Research Vols. 726-731Cloning and Sequence Analysis of Two cDNA Encoding...

Cloning and Sequence Analysis of Two cDNA Encoding Invertase Inhibitors from Cassava (Manihot esculenta Crantz)

Abstract:

In this study, two encoding invertase inhibitors (INH) named as MeINH1 and MeINH2 were isolated from cassava (Manihot esculenta Crantz). TheMeINH1 contains a 583 bp fragment in length and codes a region of 172 amino acids; and the MeINH2 contains a 538 bp fragment in length and codes a region of 169 amino acids. The amino acid sequence analysis showed that both MeINH1 and MeINH2 have typical structure of invertase inhibitor proteins with a N-terminal signal peptide, four conserved cysteine residues and two disulfide bridges, respectively; whereas, they showed low homology to other organisms. This research provides a foundation for further study of the cassava INH.

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Periodical:

Advanced Materials Research (Volumes 726-731)

Pages:

4326-4329

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.726-731.4326

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Online since:

August 2013

Authors:

Meng Ting Geng, Yuan Yao, Xiao Hui Wu, Yi Min, Shao Ping Fu, Xin Wen Hu, Jian Chun Guo

Keywords:

Cassava, Gene Cloning, Invertase Inhibitor

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