Genetic Diversity of Phalaris arundinacea Linn Germplasm Detected by SRAP Markers

Abstract:

Sequence-related amplified polymorphism (SRAP) molecular marker was used to detect the genetic diversity of 25 accessions of Phalaris arundinacea Linn that collected from the USA, Russia, Kazakhstan, Canada, LanZhou and HuBei. The following results were obtained: 1) Sixteen primer pairs produced 131 polymorphic bands, with an average of 8.19 bands per primer pair. The percentage of polymorphic bands on average was 89.25%. The polymorphism information content (PIC) ranged from 0.784 to 0.9069, with an average of 0.8696. 2) The Neis genetic similarity coefficient of the tested accessions ranged from 0.5959 to 0.8425, and the average of Neis coefficient was 0.7422. These results suggested that there was rich genetic diversity among the resources of Phalaris arundinacea Linn. 3) Twenty-five accessions were clustered into five groups. Moreover, the accessions from the same or similar origin frequently clustered into one group. The findings implied that the correlation among the resources, geographical and ecological environment.

• Periodical: Advanced Materials Research (Volumes 726-731)
• Pages: 4494-4503
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.726-731.4494
• Online since: August 2013
• Authors: Kai Kai Cheng, Juan Du, Yan Hua Huang, Casler Michael, Yun Wei Zhang
• Keywords: Genetic Diversity, Phalaris arundinacea linn , SRAP

References

[1] Barrett B, Griffiths A, Schreiber M. etal: Theoretical and Applied Genetics (2004)

[2] B. Narasimhamoorthy, M. C. Saha, T. Swaller, J. H. Bouton: Bioenerge (2008)

[3] Budak H, Shearman R C, Parmaksiz I, etal: Theoretical and Applied Geneticas (2004)

[4] Casler MD: Ecotypic variation among switchgrass populations from the Northern USA. Crop Sci (2005)

DOI: 10.2135/cropsci2005.0388

[5] Chen X , Guo H L, Xue D D: Acta Prataculturae Sinica (2009)

[6] Ferriol M, Pico B, Nuze F: Theoretical and Applied Genetics (2003)

[7] Ferriol M, Pico B, Nuez F: Genetic resources and Crop Evolution (2003)

[8] George J, Dobrowolski M P, Van-Zijll-de-Jong E, etal: Genome (2006)

[9] G. J. Vandemark, J. J. Ariss, G. A. Bauchan, R. C. Larsen, T. J. Hughes. Estimating genetic relationships among historical sources of alfalfa germplasm and selected cultivars with sequence related amplified polymorphisms: Euphytica (2006)

DOI: 10.1007/s10681-006-9167-7

[10] Grant, V: The Evolutionary Process: A Critical Study of Evolutionary Theory (Columbia University Press, New York 1991)

[11] Guo H L, Zheng Y Q, Chen X: Acta Prataculturae Sinica (2009)

[12] Han X Y, Wang L S, Shu Q Y, etal: Biochemical Genetics (2008)

[13] Hu X H, Diao Y, Zheng X F: Chinese Agricultural Science Bulletin (2010)

[14] Huang J Y, Gai S P, Zhang E Y: Chinese Agricultural Science Bulletin (2009)

[15] Kubik C, Sawkins M, Meyer WA, Gaut BS: Genetic diversity in seven perennial ryegrass (Lolium perenne L.) cultivars based on SSR markers. Crop Sci (2001)

DOI: 10.2135/cropsci2001.4151565x

[16] Li G, Quiros CF: Theoretical and Applied Genetics (2001)

[17] Li RF, Hui RK, Deng RN, Cai ML, Liu Y. : PRATACULTURAL SCIENCE (2010)

[18] Marten G C. Reed canarygras. In: Heath M E, Barnes R F, Metcalfe D S. eds: Forages: the science of grassland agriculture, 4th ed [M]. Iowa ( Iowa State University Press 1985)

[19] Nick Prasser and Joy B. Zedle: Salt Tolerance of Invasive Phalaris arundinacea Exceeds That of Native Carex stricta (Wisconsin). ECOLOGICAL RESTORATION (2010)

DOI: 10.3368/er.28.3.238

[20] Noah J. Jenkins, J.AlanYeakley, Elaine M. Stewart: FIRST-YEAR RESPONSES TO MANAGED FLOODING OF LOWER COLUMBIARIVER BOTTOMLAND VEGETATION DOMINATED BY PHALARIS ARUNDINACEA. The Society of Wetland Scientists (2008)

DOI: 10.1672/06-145.1

[21] Roldan-Ruiz I, Dendauw J, Van Bockstaele E,etal: Molecular Breeding (2000)

[22] Sahramaa M:Evaluating germplasm of reed canary grass, Phalaris arundinacea L [M]. Helsinki : (University of Helsinki 2004)

[23] Sahramaa M, HÊ mmÊL, J auhiainen L: Crop Science (2004)

[24] Sahramaa M: Agricultural and Food Science in Finland (2003)

[25] Shao YC, Xu L, Chen FS: Mycoscience (2011)

[26] Vandemark G J, Ariss J J, Bauchan G A, etal: Euphytica (2006)

[27] Wang Z Y, Yuan X J, Liu J X:Acta Prataculturae Sinica (2003)

[28] Wilson B L, Kitzmiller J, Rolle W, etal: Canadian Journal of Botanyrevue. Canadienne de Botanique(2001)

[29] Xu S B, Tao Y F, Yang Z Q: HEREDITAS (2002)

[30] Xue D D, Zheng Y Q, Wang Z Y: Acta Prataculturae Sinica (2008)

[31] Yi YJ, Zhang XQ, Huang LK: HEREDITAS (2008)

[32] Zhang JC, Wang CT, Jiao K: Chinese Agricultural Science Bulletin (2005)

[33] Zhang JY, Peng Y, Luo Y, Ma X: Acta Prataculturae Sinica (2010)

[34] Zhang YW, Juan E. Zalapa, Andrew R. Jakubowski: Genetica (2011)