The Basic Study of Plant Esterase in Pesticide Residue Examination

Abstract:

We choose three plant esterase sources, through the measurement of enzyme activity , the plant esterase that sieved efficiently withdraws the raw material, and as to it's the crude extract of the esterase to suggest the reaction condition of the liquid to carry on the system excellent turn, such as pH, reaction time and dilution. So we can guarantee the enzyme inhibition represses the method in great quantities takes out to check the sample carries on the quickly thick of accurate degree, also soon measures the card for the fixed and organ phosphorus pesticide of thorough study to provide the reference.

• Periodical: Advanced Materials Research (Volumes 726-731)
• Pages: 950-957
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.726-731.950
• Online since: August 2013
• Authors: Jing Chen, Yao Li Zhang, Yue Wu, Jiong Zhang
• Keywords: Enzyme Inhibition, Organ Phosphorus Pesticide, Plant Esterase

References

[1] HUANG Bao-Hong, Yao Yin. Esterase choice for detection of pesticide residues in plant [J]. Anhui Technical Teachers College, 2004, 18 (2):15-17.

[2] Wen Yanxia. The plant esterase screening and purification and study of pesticide sensitivity [D] Shaanxi: Shaanxi Normal University, 2006.

[3] Zhao Yongfu. Rapid detection of organophosphorus pesticide residues in vegetables research [D]. ​​Henan: Henan University, (2006)

[4] Huang Zhiyong, Yuan Yuan, Lu Yu. Chak two enzymes of organophosphorus pesticide residues in vegetables inhibit the rapid detection method comparison studies, food science, 2003, 24 (8): 135 to 137

[5] Xu Fei, Zhang Huijun, Xu Xueqin, China Zezhao. Plant esterase screening for organophosphorus pesticide residue detection [J]. Shanghai University of Technology, 2003, 25 (2):109-116.

[6] Xiao Jianjun, China Zezhao, Chen Ertong. Cooling and cold storage of wheat esterase activity of Engineering Thermophysics, 2003, 7:682 to 684

[7] Ahmadsaeed, Boeck SD, Debruyne I. Hen egg yolk cholinesterase purify-cation characterization and comparison with hen, sliver and blood plasma cholinesterase [J]. Biochimica Biophysica Acta, 1980, 61 (4): 389 ~ 399.

DOI: 10.1016/0005-2744(80)90228-4

[8] Van Asperen KA study of housefly esterases by means of a sensitive colorimetric method. [J]. J Ins.Physil, 1962, 8:401-416.

DOI: 10.1016/0022-1910(62)90074-4

[9] Zhong Shuming, Yuan Dongxing, Li Quan Long. Plant Hydrolases rapid determination of organophosphate and carbamate pesticides in environmental water samples, Xiamen University (Natural Science), 2002, 41 (1): 75 to 78

[10] Chen Fan, Chen Huanlin. Plant esterase the speed measured poisoned barnyard Residues, Environmental Science and Technology, 2005, 1:48 ~ 50

[11] Zhang Ning. Two enzymes rapid detection of organophosphorus pesticide residues Optimization of Agricultural Sciences 2006, 1:135 to 137

[12] Kang Tianfang, Zhao Hua, Zhang Yan, Zhang Kobushi. Rapid detection of organophosphorus pesticides in vegetables with Phytoesterase Beijing University of Technology, 2006, Volume 32: 1057 to 1061

[13] Shao Jia. A soybean esterase immobilized and applied research [D] Jilin: Jilin Agricultural University, (2008)

[14] Food Hygiene Supervision and Inspection of the Ministry of Health GB / T 5009.199-2003 vegetables organophosphate and carbamate pesticide residues in the rapid detection [S], Beijing: China Standard Press, (2003)