Expression and Purification of His-chIFN-α and its Antiviral Activity Assay

Abstract:

In this paper, Chicken alpha interferon (IFN-α) gene was cloned into pUC19-His expression vector, then the recombinant expression vector was transformed into host bacteria E. coli BL21. The recombinant chicken IFN-α was induced to express by IPTG, then the protein expression was analyzed with SDS-PAGE. Under the condition that the recombinant protein was induced to express with 1 mM IPTG at 37 °C, the expressed protein was inclusion body. His-chIFN-α was purified by Ni-metal chelate affinity chromatography. His-chIFN-α was shown to inhibit the replication of Newcastle disease virus in CEF cells.

• Periodical: Advanced Materials Research (Volumes 750-752)
• Pages: 1515-1519
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.750-752.1515
• Online since: August 2013
• Authors: Yue Ma*, Min Hui Long, Ai Po Diao
• Keywords: Antiviral Activity, Chicken Interferon, NDV, Prokaryotic Expression, Protein Purification

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