Salidroside Inhibits Endogenous Hydrogen Peroxide Induced Cytotoxicity of Endothelial Cells

Abstract:

To explore the protective effects of salidroside against endogenous hydrogen peroxide (H₂O₂) -induced cytotoxicity in human endothelial cells (EVC-304). EVC-304 cells were incubated in the presence or absence of low steady states of H₂O₂ (34μM) generated by glucose oxidase (GOX) with or without salidroside. MTT assays were performed, together with flow cytometric analysis using propidium (PI) label. The results indicated that salidroside could attenuate H₂O₂ induced cytotoxicity in EVC-304 cells in a dose-dependent pattern. Furthermore, flow cytometric analysis revealed that salidroside could also inhibited the G2/M arrest induced by endogenous hydrogen. The present study demonstrates that salidroside could inhibit endogenous hydrogen peroxide induced cytotoxicity of endothelial cells .

• Periodical: Advanced Materials Research (Volumes 750-752)
• Pages: 1529-1532
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.750-752.1529
• Online since: August 2013
• Authors: Xing Yu Zhao, Lian Hai Jin, Dong Jun Wang, Bin Xu, Wei Zhang, Zheng Li Luo*
• Keywords: Cytotoxicity, Hydrogen Peroxide, Salidroside

References

[1] Kanupriya, Prasad D, Sai Ram M, Kumar R, Sawhney RC, Sharma SK et al. Cytoprotective and antioxidant activity of Rhodiola imbricata against tert-butyl hydroperoxide induced oxidative injury in U-937 human macrophages. Mol Cell Biochem. 275(2005).

DOI: 10.1007/s11010-005-7637-1

[2] GAO Ming-xin, Wei-ping M. Progress of research study on pharmacological action of Rhodiola rosea. ACMP. (2008), pp.57-9.

[3] Zhang L, Yu H, Sun Y, Lin X, Chen B, Tan C et al. Protective effects of salidroside on hydrogen peroxide-induced apoptosis in SH-SY5Y human neuroblastoma cells. Eur J Pharmacol. 564(2007), pp.18-25.

DOI: 10.1016/j.ejphar.2007.01.089

[4] Yu S, Liu M, Gu X, Ding F. Neuroprotective effects of salidroside in the PC12 cell model exposed to hypoglycemia and serum limitation. Cell Mol Neurobiol. 28(2008), pp.1067-78.

DOI: 10.1007/s10571-008-9284-z

[5] Tian-Hua YAN, Bao-Sheng DU, Wei YANG, Ying MA, Jing XU, WANG Q-J. Effects of Salidroside on Hemorheology and Myocardial Ischemic Reperfusion Injury in Isolated Heart of Rats Chin J Nat Med. 6(2008), pp.219-22.

DOI: 10.3724/sp.j.1009.2008.00219

[6] Salazar JJ, Van Houten B. Preferential mitochondrial DNA injury caused by glucose oxidase as a steady generator of hydrogen peroxide in human fibroblasts. Mutat Res. 385(1997), pp.139-49.

DOI: 10.1016/s0921-8777(97)00047-5

[7] D'Agnillo F, Alayash AI. A role for the myoglobin redox cycle in the induction of endothelial cell apoptosis. Free Radic Biol Med. 33(2002), pp.1153-64.

DOI: 10.1016/s0891-5849(02)01007-9

[8] Kucinskaite A, Briedis V, Savickas A. [Experimental analysis of therapeutic properties of Rhodiola rosea L. and its possible application in medicine]. Medicina (Kaunas). 40(2004), pp.614-9.